The effect of neurotoxin MPTP administration to mice on the proteomic profile of brain isatin-binding proteins

Buneeva O.A.1, Kopylov A.T.1, Nerobkova L.N.2, Kapitsa I.G.2, Zgoda V.G.1, Medvedev A.E.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Zakusov Institute of Pharmacology, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20176304316      PubMed Id: 28862602
Year: 2017  Volume: 63  Issue: 4  Pages: 316-320
Isatin (indole-2,3-dione) is an endogenous indole found in the mammalian brain, peripheral organs and body fluids. It acts as a neuroprotector, which decreases manifestation of locomotor impairments in animal models of Parkinson's disease. A wide range of biological activity of isatin is associated with interaction of this regulator with numerous isatin-binding proteins. The aim of this study was to investigate the profile of brain isatin-binding proteins in mice with MPTP-induced Parkinsonism (90 min and seven days after administration of this neurotoxin). A single dose administration of MPTP (30 mg/kg, ip.) was accompanied by locomotor impairments in the open field test 90 min after administration; seven days after MPTP administration locomotor activity of mice significantly improved but did not reach the control level. Five independent experiments on proteomic profiling of isatin-binding proteins resulted in confident identification of 96±12 proteins. Development of MPTP-induced locomotor impairments was accompanied by a significant decrease in the number of isatin-binding proteins (63±6; n=5; p<0.01). Seven days after MPTP administration the total number of identified proteins increased and reached the control level (132±34; n=4). The profiles of isatin-binding proteins were rather specific for each group of mice: in the control group these proteins (which were not found in both groups of MPTP-treated mice) represented more than 70% of total proteins. In the case of MPTP treated mice this parameter was 60% (90 min after MPTP administration) and >82% (seven days after MPTP administration). The major changes were found in the groups of isatin-binding proteins involved into cytoskeleton formation and exocytosis, regulation of gene expression, cell division and differentiation and also proteins involved in signal transduction.
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Keywords: MPTP-induced Parkinsonism, isatin, isatin-binding proteins, brain, proteomic profiling
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Buneeva, O. A., Kopylov, A. T., Nerobkova, L. N., Kapitsa, I. G., Zgoda, V. G., Medvedev, A. E. (2017). The effect of neurotoxin MPTP administration to mice on the proteomic profile of brain isatin-binding proteins. Biomeditsinskaya Khimiya, 63(4), 316-320.
This paper is also available as the English translation: 10.1134/S1990750818010043
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