Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV

Bespyatykh J.A.1 , Manicheva O.A.2, Smolyakov A.V.3, Dogonadze M.Z.2, Zhuravlev V.Yu.2, Shitikov E.A.1, Ilina E.N.1

1. Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russia
2. Research Institute of Phtisiopulmonology, St. Petersburg, Russia
3. Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Russia
DOI: 10.18097/PBMC20176304334      PubMed Id: 28862605
Year: 2017  Volume: 63  Issue: 4  Pages: 334-340
Comparative proteomic profiling of M. tuberculosis H37Rv strains cultured on two different nutrient media, Levenstein-Jensen and Middlebrook 7H11, was performed using a label-free LC-MS/MS approach. It was shown that results obtained from two media possessed high convergence. The only difference was observed in the representation of fumarate reductase FrdB, its abundance was higher in the mycobacterial cells cultured on Levenstein-Jensen medium. The correlation analysis of biological repeats revealed the high convergence of the results obtained from Middlebrook 7H11 medium. Thus, we can conclude that the use of the Middlebrook 7H11 medium is most appropriate in the scientific laboratory.
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Bespyatykh J.A., Manicheva O.A., Smolyakov A.V., Dogonadze M.Z., Zhuravlev V.Yu., Shitikov E.A., Ilina E.N. (2017) Biomeditsinskaya khimiya, 63(4), 334-340.
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