Multiomics study of HepG2 cell line proteome

   
Poverennaya E.V.1, Kiseleva O.I.1 , Ponomarenko E.A.1, Naryzhny S.N.1, Zgoda V.G.1, Lisitsa A.V.1

1. Institute of Biomedical Chemistry, Moscow, Russia
Section: OMICS-Technologies
DOI: 10.18097/PBMC20176305373      PubMed Id: 29080867
Year: 2017  Volume: 63  Issue: 5  Pages: 373-378
Current proteomic studies are generally focused on the most abundant proteoforms encoded by canonical nucleic sequences. Transcriptomic and proteomic data, accumulated in a variety of postgenome sources and coupled with state-of-art analytical technologies, allow to start the identification of aberrant (non-canonical) proteoforms. The main sources of aberrant proteoforms are alternative splicing, single nucleotide polymorphism, and post-translational modifications. The aim of this work was to estimate the heterogeneity of HepG2 proteome. We suggested multiomics approach, which combines transcriptomic (RNAseq) and proteomic (2DE-MS/MS) methods, as a promising strategy to explore the proteome.
Download PDF:
Keywords: proteome, transcriptome, transcriptoproteome, proteoform, alternative splicing, single amino acid polymorphism, post-translational modification
Citation:

Poverennaya, E. V., Kiseleva, O. I., Ponomarenko, E. A., Naryzhny, S. N., Zgoda, V. G., Lisitsa, A. V. (2017). Multiomics study of HepG2 cell line proteome. Biomeditsinskaya khimiya, 63(5), 373-378.
References  
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)