Current proteomic studies are generally focused on the most abundant proteoforms encoded by canonical nucleic sequences. Transcriptomic and proteomic data, accumulated in a variety of postgenome sources and coupled with state-of-art analytical technologies, allow to start the identification of aberrant (non-canonical) proteoforms. The main sources of aberrant proteoforms are alternative splicing, single nucleotide polymorphism, and post-translational modifications. The aim of this work was to estimate the heterogeneity of HepG2 proteome. We suggested multiomics approach, which combines transcriptomic (RNAseq) and proteomic (2DE-MS/MS) methods, as a promising strategy to explore the proteome.
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Keywords: proteome, transcriptome, transcriptoproteome, proteoform, alternative splicing, single amino acid polymorphism, post-translational modification
Citation:
Poverennaya E.V., Kiseleva O.I., Ponomarenko E.A., Naryzhny S.N., Zgoda V.G., Lisitsa A.V. (2017) Multiomics study of HepG2 cell line proteome. Biomeditsinskaya Khimiya, 63(5), 373-378.
Poverennaya E.V. et al. Multiomics study of HepG2 cell line proteome // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 5. - P. 373-378.
Poverennaya E.V. et al., "Multiomics study of HepG2 cell line proteome." Biomeditsinskaya Khimiya 63.5 (2017): 373-378.
Poverennaya, E. V., Kiseleva, O. I., Ponomarenko, E. A., Naryzhny, S. N., Zgoda, V. G., Lisitsa, A. V. (2017). Multiomics study of HepG2 cell line proteome. Biomeditsinskaya Khimiya, 63(5), 373-378.
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