1. Institute of Ecology and Genetics of Microorganisms UB RAS, Perm, Russia; Perm State National Research University, Perm, Russia 2. Kant Baltic federal university, Kaliningrad, Russia 3. Institute of Ecology and Genetics of Microorganisms UB RAS, Perm, Russia 4. Perm State National Research University, Perm, Russia
The effects of chorionic gonadotropin (hCG) on the expression of the hTERT gene in combination with the conversion of the phenotype of naive T-cells and T-cells of immune memory in vitro were studied. hCG inhibited expression of hTERT mRNA in naive T-cells (CD45RA+) and immune memory T cells (CD45RO+), causing a decrease in the replicative potential of the cells. The presence of hCG in the culture led to the conversion of the phenotype of T-lymphocytes. hCG reduced the number of proliferating T-cells of immune memory, estimated by phenotypic signs by differential gating. hCG (10 IU/ml and 100 IU/ml) inhibited expression of CD25 by the studied populations, but did not modulate expression of the CD71 proliferation marker. Thus, hCG inhibited the functional activity of naive T-cells and T-cells of immune memory, which, in the context of pregnancy, can contribute to the formation of immune tolerance to the semi-allogenic fetus.
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Keywords: human chorionic gonadotropin (HCG), naive T cells, memory T-cells, CD71, CD25, hTERT, proliferation
Citation:
Rayev M.B., Zamorina S.A., Litvinova L.S., Yurova K.A., Khaziakhmatova O.G., Timganova V.P., Bochkova M.S., Kropaneva M.D., Khramtsov P.V. (2017) The influence of chorionic gonadotropin on phenotype conversion and hTERT gene expression by T-lymphocytes of different degrees of differentiation. Biomeditsinskaya Khimiya, 63(6), 539-545.
Rayev M.B. et al. The influence of chorionic gonadotropin on phenotype conversion and hTERT gene expression by T-lymphocytes of different degrees of differentiation // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 6. - P. 539-545.
Rayev M.B. et al., "The influence of chorionic gonadotropin on phenotype conversion and hTERT gene expression by T-lymphocytes of different degrees of differentiation." Biomeditsinskaya Khimiya 63.6 (2017): 539-545.
Rayev, M. B., Zamorina, S. A., Litvinova, L. S., Yurova, K. A., Khaziakhmatova, O. G., Timganova, V. P., Bochkova, M. S., Kropaneva, M. D., Khramtsov, P. V. (2017). The influence of chorionic gonadotropin on phenotype conversion and hTERT gene expression by T-lymphocytes of different degrees of differentiation. Biomeditsinskaya Khimiya, 63(6), 539-545.
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