The influence of enzymatic removal of chlorpyrifos from feed grain-mixture on the biochemical parameters of rat blood

Maslova O.V.1, Senko O.V.1, Efremenko E.N.1

1. Lomonosov Moscow State University, Faculty of Chemistry, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20176306559      PubMed Id: 29251619
Year: 2017  Volume: 63  Issue: 6  Pages: 559-564
Organophosphorus pesticides (OP) are used to protect crops from pests. Treatment of plants and animals with pesticides can be done during their growth or creation of conditions necessary for the long-shelf life of the agricultural products. Currently, there are many remedies for prevention and removal of intoxication consequences developed under the action of OP in living organisms. The development of biologics for the degradation of OP and biotechnologies for their application in agriculture is relevant. New biologics based on the stabilized forms of such enzyme as hexahistidine-tagged organophosphorus hydrolase (His6-OPH) in the form of nano-sized particles were tried for OP detoxification. These biologics (enzyme-polyelectrolyte complexes, EPC) were obtained in accordance to previously developed procedure by mixing solutions of His6-OPH and polyanion under certain conditions. The main purpose of this work was to evaluate the usage efficiency of EPC based on His6-OPH and polyglutamic acid for OP detoxification by analyzing biochemical blood parameters of rats consumed the grain-mixture containing chlorpyrifos. The experiment was conducted using female Sprague Dawley albino rats. Treatment of feeding grain-mixture initially containing chlopyrifos (48 mg/kg of the mixture) with EPC based on His6-OPH (1000 U/kg of the mixture) for 24 h was the most effective. The results showed that rats from the group consuming food after enzymatic removal of chlorpyrifos, had comparable acetyl cholinesterase activity in blood of rats consuming pure food (without any OP intoxication).
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Keywords: hexahistidine-tagged organophosphorus hydrolase, organophosphorus pesticides, hydrolysis, biochemical blood analysis

Maslova, O. V., Senko, O. V., Efremenko, E. N. (2017). The influence of enzymatic removal of chlorpyrifos from feed grain-mixture on the biochemical parameters of rat blood. Biomeditsinskaya khimiya, 63(6), 559-564.
This paper is also available as the English translation: 10.1134/S1990750818020087
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