Donor chimerism and minimal residual disease monitoring in leukemia patients after allo-HSCT

Bogdanov K.V.1 , Motorin D.V.1, Nikulina T.S.1, Pisotskaya O.S.1, Babenetskaya D.V.1, Mirolyubova Y.V.1, Volkova O.Y.1, Zaritskiy A.Y.1

1. Institute of Hematology, Federal V.A. Almazov North-West Medical Research Centre, St. Petersburg, Russia
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20176306570      PubMed Id: 29251621
Year: 2017  Volume: 63  Issue: 6  Pages: 570-581
In present research the comparative analysis of donor chimerism (DC) using different tests was performed to improve the diagnostic tool in patients with malignant hematological disorders after allo-HSCT. The RBC antigen typing, identification of ABO blood type and quantitative analysis of InDel-, STR-, Y-polymorphisms were carried out for detection of DC. In addition, the expression of well-known oncogenes and CD-markers for monitoring MRD was evaluated to predict relapse and clinical outcome. According to our research, the analysis of InDel polymorphism using AlleleSEQR-PCR is more sensitive test for estimation of DC as compared with other assays. Moreover, the sensitivity of AlleleSEQR-PCR may be increased after isolation of the CD34 cell population in bone marrow. Nevertheless, observation of high levels in DC (³95%) in some leukemia patients (ALL, Ph+, bcr-abl/p190+) during first 6 months after HSCT cannot exclude the possibility of relapse. Thus, the combined monitoring of both DC (InDel) and MRD (oncogenes, WT1 and CD-markers) is a more advisable and useful test in managing hematologic malignancies and predicting relapse risk after allo-HSCT.
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Keywords: leukemia, transplantation, monitoring, donor chimerism, MRD

Bogdanov, K. V., Motorin, D. V., Nikulina, T. S., Pisotskaya, O. S., Babenetskaya, D. V., Mirolyubova, Y. V., Volkova, O. Y., Zaritskiy, A. Y. (2017). Donor chimerism and minimal residual disease monitoring in leukemia patients after allo-HSCT. Biomeditsinskaya Khimiya, 63(6), 570-581.
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