1. Immanuel Kant Baltic Federal University, Kaliningrad, Russia 2. Immanuel Kant Baltic Federal University, Kaliningrad, Russia; Regional Clinical Hospital of the Kaliningrad Region, Kaliningrad, Russia 3. Regional Clinical Hospital of the Kaliningrad Region, Kaliningrad, Russia
Chemerin is a mediator of adipose tissue involved in the regulation of many processes, including lipogenesis, and inflammatory response. The role of chemerin in the development of insulin resistance has been insufficiently studied and needs detailed understanding. The aim of the study was to investigate chemerin production in obese patients with different states of carbohydrate metabolism. The study included 155 patients with a diagnosis of obesity; 34 patients with overweight. The control group 1 consisted of 43 conditionally healthy donors who did not have obesity. For comparison of the results of a study to determine the levels of tissue-specific mRNA expression of the genes IL-6, TNF-a, RARRES2, (encoding IL-6, TNF-a and chemerin) in adipose tissue introduced a control group 2 – 30 patients without obesity. Study on the relative level of mRNA expression of the genes IL-6, TNF-a and RARRES2 (encoding IL-6, TNF-a and chemerin) was carried out using real time PCR. Concentrations of IL-6, TNF-a, and chemerin were measured in serum/plasma using an enzyme-linked immunosorbent assay (ELISA). We found significant differences in the plasma level of chemerin and tissue-specific features of RARRES2 gene expression in obese patients, depending on the degree of obesity and the state of carbohydrate metabolism. Multidirectional associations of RARRES2 gene expression with TNF-a and IL-6 genes in adipose tissues of different localization are shown: in obese patients (BMI £40 kg/m2) without type 2 diabetes – negative, and type 2 diabetes – positive. Identified relationship chemerin plasma content and the expression level of its gene in biopsies with various parameters of carbohydrate and lipid metabolism, proinflammatory molecules indicate chemerin involved in metabolic and immune processes in obesity.
Vasilenko M.A., Kirienkova E.V., Skuratovskaya D.A., Zatolokin P.A., Mironyuk N.I., Litvinova L.S. (2017) The chemerin production changes in obese patients with different carbohydrate metabolism state. Biomeditsinskaya Khimiya, 63(6), 582-590.
Vasilenko M.A. et al. The chemerin production changes in obese patients with different carbohydrate metabolism state // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 6. - P. 582-590.
Vasilenko M.A. et al., "The chemerin production changes in obese patients with different carbohydrate metabolism state." Biomeditsinskaya Khimiya 63.6 (2017): 582-590.
Vasilenko, M. A., Kirienkova, E. V., Skuratovskaya, D. A., Zatolokin, P. A., Mironyuk, N. I., Litvinova, L. S. (2017). The chemerin production changes in obese patients with different carbohydrate metabolism state. Biomeditsinskaya Khimiya, 63(6), 582-590.
Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., Speleman F. (2002) Genome Biol., 3(7), research0034.1- research0034.11. CrossRef Scholar google search
Zylla S., Pietzner M., Kühn J.P., Völzke H., Dörr M., Nauck M., Friedrich N. (2017) Obesity (Silver Spring), 25, 468-475. CrossRef Scholar google search
Chakaroun R.I., Raschpichler M., Klöting N., Oberbach A., Flehmig G., Kern M., Schön M.R., Shang E., Lohmann T., Dreßler M., Fasshauer M., Stumvoll M., Blüher M. (2012) Metabolism, 61, 706-714. CrossRef Scholar google search
Takahashi M., Inomata S., Okimura Y., Iguchi G., Fukuoka H., Miyake K., Koga D., Akamatsu S., Kasuga M., Takahashi Y. (2013) Endocr. J., 60, 37-44. CrossRef Scholar google search
Shin H.Y., Lee D.C., Chu S.H., Jeon J.Y., Lee M.K., Im J.A., Lee J.W. (2012) Clin. Endocrinol., 77, 47-50. CrossRef Scholar google search
Alfadda A.A., Sallam R.M., Chishti M.A., Moustafa A.S., Fatma S., Alomaim W.S., Al-Naami M.Y., Bassas A.F., Chrousos G.P., Jo H. (2012) Mol. Cells, 33, 591-596. CrossRef Scholar google search
Sledzinski T., Korczynska J., Hallmann A., Kaska L., Proczko-Markuszewska M., Stefaniak T., Sledzinski M., Swierczynski J. (2013) J. Endocrinol. Invest., 36, 428-434. Scholar google search
Takahashi M., Takahashi Y., Takahashi K., Zolotaryov F.N., Hong K.S., Kitazawa R., Iida K., Okimura Y., Kaji H., Kitazawa S., Kasuga M., Chihara K. (2008) FEBS Lett., 582, 573-578. CrossRef Scholar google search
Kaur J., Adya R., Tan B.K., Chen J., Randeva H.S. (2010) Biochem. Biophys. Res. Commun., 391, 1762-1768. CrossRef Scholar google search
Neves K.B., Nguyen Dinh Cat A., Lopes R.A., Rios F.J., Anagnostopoulou A., Lobato N.S., de Oliveira A.M., Tostes R.C., Montezano A.C., Touyz R.M. (2015) Hypertension, 66, 657-666. CrossRef Scholar google search
Pfau D., Bachmann A., Lössner U., Kratzsch J., Blüher M., Stumvoll M., Fasshauer M. (2010) DiabetesCare, 33, 171-173. CrossRef Scholar google search
Weigert J., Neumeier M., Wanninger J., Filarsky M., Bauer S., Wiest R., Farkas S., Scherer M.N., Schäffler A., Aslanidis C., Schölmerich J., Buechler C. (2010) Clin. Endocrinol. (Oxf.), 72, 342-348. CrossRef Scholar google search
Parlee S.D., Ernst M.C., Muruganandan S., Sinal C.J., Goralski K.B. (2010) Endocrinology, 151, 2590-2602. CrossRef Scholar google search
