The chemerin production changes in obese patients with different carbohydrate metabolism state

   
Vasilenko M.A.1, Kirienkova E.V.1, Skuratovskaya D.A.1, Zatolokin P.A.2, Mironyuk N.I.3, Litvinova L.S.1

1. Immanuel Kant Baltic Federal University, Kaliningrad, Russia
2. Immanuel Kant Baltic Federal University, Kaliningrad, Russia; Regional Clinical Hospital of the Kaliningrad Region, Kaliningrad, Russia
3. Regional Clinical Hospital of the Kaliningrad Region, Kaliningrad, Russia
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20176306582      PubMed Id: 29251622
Year: 2017  Volume: 63  Issue: 6  Pages: 582-590
Chemerin is a mediator of adipose tissue involved in the regulation of many processes, including lipogenesis, and inflammatory response. The role of chemerin in the development of insulin resistance has been insufficiently studied and needs detailed understanding. The aim of the study was to investigate chemerin production in obese patients with different states of carbohydrate metabolism. The study included 155 patients with a diagnosis of obesity; 34 patients with overweight. The control group 1 consisted of 43 conditionally healthy donors who did not have obesity. For comparison of the results of a study to determine the levels of tissue-specific mRNA expression of the genes IL-6, TNF-a, RARRES2, (encoding IL-6, TNF-a and chemerin) in adipose tissue introduced a control group 2 – 30 patients without obesity. Study on the relative level of mRNA expression of the genes IL-6, TNF-a and RARRES2 (encoding IL-6, TNF-a and chemerin) was carried out using real time PCR. Concentrations of IL-6, TNF-a, and chemerin were measured in serum/plasma using an enzyme-linked immunosorbent assay (ELISA). We found significant differences in the plasma level of chemerin and tissue-specific features of RARRES2 gene expression in obese patients, depending on the degree of obesity and the state of carbohydrate metabolism. Multidirectional associations of RARRES2 gene expression with TNF-a and IL-6 genes in adipose tissues of different localization are shown: in obese patients (BMI £40 kg/m2) without type 2 diabetes – negative, and type 2 diabetes – positive. Identified relationship chemerin plasma content and the expression level of its gene in biopsies with various parameters of carbohydrate and lipid metabolism, proinflammatory molecules indicate chemerin involved in metabolic and immune processes in obesity.
Download PDF:
Keywords: insulin resistance, obesity, type 2 diabetes mellitus, chemerin, mRNA, PCR
Citation:

Vasilenko, M. A., Kirienkova, E. V., Skuratovskaya, D. A., Zatolokin, P. A., Mironyuk, N. I., Litvinova, L. S. (2017). The chemerin production changes in obese patients with different carbohydrate metabolism state. Biomeditsinskaya khimiya, 63(6), 582-590.
References  
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)