OMERO service was used to annotate the cell line HaCaT microscope images by two independent expert groups. The images were obtained in the course of developing tissue-engineered epithelium which consisted of several layers of the keratinocytes. Evaluation of expert opinions was performed by calculation of specificity, sensitivity and accuracy. The best convergence of opinions (91%) was achieved for the confluence of the cell monolayers. Accuracy 70% was observed in determining the extent of cell differentiation after 10 days of incubation. The paper illustrates the usefulness of OMERO service for dynamic cross-validation of quality in the development and standardization of cell preparations.
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Keywords: OMERO, skin model, reconstructed human epidermis, HaCaT keratinocytes
Citation:
Nakhod K.V., Rusanov A.L., Luzgina E.D., Druzhilovskiy D.S., Luzgina N.G., Lisitsa A.V. (2018) Quality control study of engineered skin tissue. Biomeditsinskaya Khimiya, 64(1), 10-15.
Nakhod K.V. et al. Quality control study of engineered skin tissue // Biomeditsinskaya Khimiya. - 2018. - V. 64. -N 1. - P. 10-15.
Nakhod K.V. et al., "Quality control study of engineered skin tissue." Biomeditsinskaya Khimiya 64.1 (2018): 10-15.
Nakhod, K. V., Rusanov, A. L., Luzgina, E. D., Druzhilovskiy, D. S., Luzgina, N. G., Lisitsa, A. V. (2018). Quality control study of engineered skin tissue. Biomeditsinskaya Khimiya, 64(1), 10-15.
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