Quality control study of engineered skin tissue

   
Nakhod K.V.1, Rusanov A.L.2 , Luzgina E.D.2, Druzhilovskiy D.S.1, Luzgina N.G.1, Lisitsa A.V.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Research and Manufacturing Association “Perspectiva”, Novosibirsk, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20186401010      PubMed Id: 29460829
Year: 2018  Volume: 64  Issue: 1  Pages: 10-15
OMERO service was used to annotate the cell line HaCaT microscope images by two independent expert groups. The images were obtained in the course of developing tissue-engineered epithelium which consisted of several layers of the keratinocytes. Evaluation of expert opinions was performed by calculation of specificity, sensitivity and accuracy. The best convergence of opinions (91%) was achieved for the confluence of the cell monolayers. Accuracy 70% was observed in determining the extent of cell differentiation after 10 days of incubation. The paper illustrates the usefulness of OMERO service for dynamic cross-validation of quality in the development and standardization of cell preparations.
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Keywords: OMERO, skin model, reconstructed human epidermis, HaCaT keratinocytes
Citation:

Nakhod, K. V., Rusanov, A. L., Luzgina, E. D., Druzhilovskiy, D. S., Luzgina, N. G., Lisitsa, A. V. (2018). Quality control study of engineered skin tissue. Biomeditsinskaya khimiya, 64(1), 10-15.
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