1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia 2. Tomsk Cancer Research Institute, Tomsk, Russia 3. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia
To increase the sensitivity and specificity of the developed methods for diagnosis of oncological diseases using exosomes of blood, a stage of pre-selection of tumor exosomes from a common pool of circulating microvesicles is required. In the present work, universal proteins have been identified, their expression has been increased in the exosomes of patients with colorectal cancer, head and neck squamous cell carcinomas, and lung cancer. The use of antibodies against major exosomal proteins will further develop a simple and high-performance method of affinity isolation of tumor exosomes.
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Keywords: exosomes, transmission electron microscopy, flow cytometry, proteins, colorectal cancer, head and neck squamous cell carcinoma, lung cancer
Citation:
Tamkovich S.N., Yunusova N.V., Somov A.K., Kakurina G.V., Kolegova E.S., Tugurova E.A., Laktionov P.P., Kondakova I.V. (2018) Comparative sub-population analysis of exosomes from blood plasma of cancer patients. Biomeditsinskaya Khimiya, 64(1), 110-114.
Tamkovich S.N. et al. Comparative sub-population analysis of exosomes from blood plasma of cancer patients // Biomeditsinskaya Khimiya. - 2018. - V. 64. -N 1. - P. 110-114.
Tamkovich S.N. et al., "Comparative sub-population analysis of exosomes from blood plasma of cancer patients." Biomeditsinskaya Khimiya 64.1 (2018): 110-114.
Tamkovich, S. N., Yunusova, N. V., Somov, A. K., Kakurina, G. V., Kolegova, E. S., Tugurova, E. A., Laktionov, P. P., Kondakova, I. V. (2018). Comparative sub-population analysis of exosomes from blood plasma of cancer patients. Biomeditsinskaya Khimiya, 64(1), 110-114.
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