Possibility of application of extracellular protease of micromycet Aspergillus ochraceus VKM F-4104D for determination of protein C content in human blood plasma

   
Osmolovskiy A.A.1 , Orekhova A.V.2, Kreyer V.G.1, Baranova N.A.1, Egorov N.S.3

1. Biological Faculty of Lomonosov Moscow State University, Moscow, Russia
2. Biological Faculty of Lomonosov Moscow State University, Moscow, Russia; Faculty of Fundamental Medicine of Lomonosov Moscow State University, Moscow, Russia
3. International Biotechnology Center of Lomonosov Moscow State University, Moscow, Russia
Section: Review
DOI: 10.18097/PBMC20186401115      PubMed Id: 29460841
Year: 2018  Volume: 64  Issue: 1  Pages: 115-118
It was shown that the activator activity of protein C, determined in normal plasma using Aspergillus ochraceus protease, is comparable with the activity of commercial protease analogue from the South American copperhead venom (ProtacÒ). It was found that protease of A. ochraceus can be used to determine protein C in plasma with its reduced content similar to ProtacÒ. Comparison of the activator protein C activity of A. ochraceus protease and the commercial analogue showed some excess of the activator activity of the fungal preparation, which may make it a promising substitute for the snake activator in diagnostical kits for determining the protein C content in clinical laboratories.
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Keywords: proteases of micromycetes, activators of protein C, diagnosis of protein C
Citation:

Osmolovskiy, A. A., Orekhova, A. V., Kreyer, V. G., Baranova, N. A., Egorov, N. S. (2018). Possibility of application of extracellular protease of micromycet Aspergillus ochraceus VKM F-4104D for determination of protein C content in human blood plasma. Biomeditsinskaya khimiya, 64(1), 115-118.
This paper is also available as the English translation: 10.1134/S1990750818020099
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