1. Belarusian State University, Minsk, Belarus 2. Institute for Experimental Medicine, Saint-Petersburg, Russia; Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia 3. Institute for Experimental Medicine, Saint-Petersburg, Russia; Saint Petersburg University, Saint-Petersburg, Russia 4. Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia; Pirogov Russian National Research Medical University, Moscow, Russia 5. Institute for Experimental Medicine, Saint-Petersburg, Russia; Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia; Saint Petersburg University, Saint-Petersburg, Russia; Centre of Preclinical Translational Research, Almazov National Medical Research Centre, Saint-Petersburg, Russia
Exocytosis of myeloperoxidase (MPO) from activated neutrophils in the presence of the anionic polysaccharide heparin was studied. It was determined that the optimal concentration of heparin (0.1 u/ml), at which there is no additional activation of cells (absence of amplification of exocytosis of lysozyme contained in specific and azurophilic granules). It was found that after preincubation of cells with heparin (0.1 u/ml) the exocytosis of MPO from neutrophils activated by various stimulants (fMLP, PMA, plant lectins CABA and PHA-L) increased compared to that under the action of activators alone. In addition, it was shown that heparin in the range of concentrations 0.1-50 u/ml did not affect on the peroxidase activity of the MPO isolated from leukocytes. Thus, the use of heparin at a concentration of 0.1 u/ml avoids the artifact caused by the “loss” of MPO in a result of its binding to neutrophils, and increases the accuracy of the method of registration the degranulation of azurophilic granules of neutrophils based on determination of the concentration or peroxidase activity of MPO in cell supernatants.
Grigorieva D.V., Gorudko I.V., Kostevich V.A., Vasilyev V.B., Cherenkevich S.N., Panasenko O.M., Sokolov A.V. (2018) Exocytosis of myeloperoxidase from activated neutrophils in the presence of heparin. Biomeditsinskaya Khimiya, 64(1), 16-22.
Grigorieva D.V. et al. Exocytosis of myeloperoxidase from activated neutrophils in the presence of heparin // Biomeditsinskaya Khimiya. - 2018. - V. 64. -N 1. - P. 16-22.
Grigorieva D.V. et al., "Exocytosis of myeloperoxidase from activated neutrophils in the presence of heparin." Biomeditsinskaya Khimiya 64.1 (2018): 16-22.
Grigorieva, D. V., Gorudko, I. V., Kostevich, V. A., Vasilyev, V. B., Cherenkevich, S. N., Panasenko, O. M., Sokolov, A. V. (2018). Exocytosis of myeloperoxidase from activated neutrophils in the presence of heparin. Biomeditsinskaya Khimiya, 64(1), 16-22.
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