1. Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia 2. Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia; Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia 3. Novosibirsk State University, Novosibirsk, Russia 4. FSBIH SDMC of FMBA of Russia, Novosibirsk, Russia 5. Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia
Urine of prostate cancer patients contains tumor-specific biopolymers, including protein- and microvesiclesassociated miRNAs that can potentially be used as oncomarkers. Previously we have characterized urine extracellular vesicles and demonstrated diagnostic potential of their miRNA cargo. In this study, we have performed a comparative analysis of the expression of 84 miRNA in paired samples of urine microvesicles and clarified urine from healthy men, patients with benign hyperplasia and cancer of the prostate using miRCURY LNA miRNA qPCR Panels. Subsets of miRNAs with differences in expression between the fractions of the urine were found in all three groups. Two groups of miRNA were identified based on the patterns of their differential expression. They regulate several key signaling pathways associated with prostate cancer development.
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Keywords: miRNA, exosomes, microvesicles, urine, prostate cancer
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Zaporozhchenko I.A., Bryzgunova O.E., Lekchnov E.A., Osipov I.D., Zaripov M.M., Yurchenko Yu.B., Yarmoschuk S.V., Pashkovskaya O.A., Rykova E.Yu., Zheravin A.A., Laktionov P.P. (2018) Representation analysis of miRNA from clarified urine and urine microvesicles in prostate malignancies and non-malignant neoplasms. Biomeditsinskaya Khimiya, 64(1), 38-45.
Zaporozhchenko I.A. et al. Representation analysis of miRNA from clarified urine and urine microvesicles in prostate malignancies and non-malignant neoplasms // Biomeditsinskaya Khimiya. - 2018. - V. 64. -N 1. - P. 38-45.
Zaporozhchenko I.A. et al., "Representation analysis of miRNA from clarified urine and urine microvesicles in prostate malignancies and non-malignant neoplasms." Biomeditsinskaya Khimiya 64.1 (2018): 38-45.
Zaporozhchenko, I. A., Bryzgunova, O. E., Lekchnov, E. A., Osipov, I. D., Zaripov, M. M., Yurchenko, Yu. B., Yarmoschuk, S. V., Pashkovskaya, O. A., Rykova, E. Yu., Zheravin, A. A., Laktionov, P. P. (2018). Representation analysis of miRNA from clarified urine and urine microvesicles in prostate malignancies and non-malignant neoplasms. Biomeditsinskaya Khimiya, 64(1), 38-45.
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