Construction and characterization of a recombinant mutant homolog of the CheW protein from Thermotoga petrophila RKU-1

   
Grishin D.V.1 , Zhdanov D.D.1, Gladilina Ju.A.1, Pokrovsky V.S.1, Podobed O.V.1, Pokrovskaya M.V.1, Aleksandrova S.S.1, Milyushkina A.L.1, Vigovskiy M.A.1, Sokolov N.N.1

1. Institute of Biomedical Chemistry, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20186401053      PubMed Id: 29460835
Year: 2018  Volume: 64  Issue: 1  Pages: 53-60
In the work a recombinant chemotaxis protein CheW from Thermotoga petrophila RKU-1 (TpeCheW) and its mutant homolog (TpeCheW-mut) were created. It was shown that, despite the low homology with CheW prototypes from intestinal bacteria, these proteins didn't cause metabolic overload and were well expressed by cells of E. coli laboratory strains. We have discovered a broad spectrum of industrial valuable properties of the TpeCheW-mut protein such as stability in a wide range of temperatures and pH, high expression level, solubility and possibility of the application of a simple low-stage purification methodology with the use of preliminary heat treatment. Possible directions of the scientific and industrial application of this protein were claimed.
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Keywords: hyperthermophilic microorganism, recombinant thermostable protein CheW, protein overexpression, biotechnology
Citation:

Grishin, D. V., Zhdanov, D. D., Gladilina, Ju. A., Pokrovsky, V. S., Podobed, O. V., Pokrovskaya, M. V., Aleksandrova, S. S., Milyushkina, A. L., Vigovskiy, M. A., Sokolov, N. N. (2018). Construction and characterization of a recombinant mutant homolog of the CheW protein from Thermotoga petrophila RKU-1. Biomeditsinskaya khimiya, 64(1), 53-60.
This paper is also available as the English translation: 10.1134/S1990750818020051
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