Methods for determining of cytochrome P450 isozymes functional activity

   
Kuzikov A.V.1, Masamrekh R.A.1, Archakov A.I.1, Shumyantseva V.V.1

1. Institute of Biomedical Chemistry, Moscow, Russia; Pirogov Russian National Research Medical University (RNRMU), Moscow, Russia
Section: Review
DOI: 10.18097/PBMC20186402149      PubMed Id: 29723145
Year: 2018  Volume: 64  Issue: 2  Pages: 149-168
The review is dedicated to modern methods and technologies for determining of cytochrome P450 isozymes functional activity, such as absorbance and fluorescent spectroscopy, electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR), Raman, Mossbauer, and X-ray spectroscopy, surface plasmon resonance (SPR), atomic force microscopy (AFM). Methods of molecular genetic analysis were reviewed from personalized medicine point of view. The use of chromate-mass-spectrometric methods for cytochrome P450-dependent catalytic reactions' products was discussed. The review covers modern electrochemical systems based on cytochrome P450 isozymes for their catalytic activity analysis, their use in practice and further development perspectives for experimental pharmacology, biotechnology and translational medicine.
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Keywords: cytochrome P450, substrates, inhibitors, electrochemical analysis, spectroscopy, mass-spectrometry
Citation:

Kuzikov, A. V., Masamrekh, R. A., Archakov, A. I., Shumyantseva, V. V. (2018). Methods for determining of cytochrome P450 isozymes functional activity. Biomeditsinskaya Khimiya, 64(2), 149-168.
This paper is also available as the English translation: 10.1134/S1990750818030046
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