The analysis of participation of individual proteins in the protein interactome formation

Florinskaya A.V.1 , Ershov P.V.1, Mezentsev Y.V.1, Kaluzhskiy L.A.1, Yablokov E.O.1, Buneeva O.A.1, Zgoda V.G.1, Medvedev A.E.1, Ivanov A.S.1

1. Institute of Biomedical Chemistry, Moscow, Russia
DOI: 10.18097/PBMC20186402169      PubMed Id: 29723146
Year: 2018  Volume: 64  Issue: 2  Pages: 169-174
It becomes increasingly clear that most proteins of living systems exist as components of various protein complexes rather than individual molecules. The use of various proteomic techniques significantly extended our knowledge not only about functioning of individual complexes but also formed a basis for systemic analysis of protein-protein interactions. In this study gel-filtration chromatography accompanied by mass-spectrometry was used for the interactome analysis of human liver proteins. In six fractions (with average molecular masses of 45 kDa, 60 kDa, 85 kDa, 150 kDa, 250 kDa, and 440 kDa) 797 proteins were identified. In dependence of their distribution profiles in the fractions, these proteins could be subdivided into four groups: (1) single monomeric proteins that are not involved in formation of stable protein complexes; (2) proteins existing as homodimers or heterodimers with comparable partners; (3) proteins that are partially exist as monomers and partially as components of protein complexes; (4) proteins that do not exist in the monomolecular state, but also exist within protein complexes containing three or more subunits. Application of this approach to known isatin-binding proteins resulted in identification of proteins involved in formation of the homo- and heterodimers and mixed protein complexes.
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Keywords: protein-protein interactions, protein interactomics, chromatographic fractionation of biological material, protein mass spectrometry, systems analysis

Florinskaya, A. V., Ershov, P. V., Mezentsev, Y. V., Kaluzhskiy, L. A., Yablokov, E. O., Buneeva, O. A., Zgoda, V. G., Medvedev, A. E., Ivanov, A. S. (2018). The analysis of participation of individual proteins in the protein interactome formation. Biomeditsinskaya Khimiya, 64(2), 169-174.
This paper is also available as the English translation: 10.1134/S1990750818030034
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