1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia 2. Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia 3. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia 4. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia
The approach for the quick and efficient production ofpolyclonal antibodies tothe target antigen alpha-synuclein has been proposed. Two methods have been employed to purify specific rabbit polyclonal antibodies against recombinant human alpha-synuclein, produced by subcutaneous immunization with complete Freund's adjuvant. It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Two-stage antibody purification procedure first on Sepharose with immobilized protein G, and then on alpha-synuclein immobilized column helps to avoid contamination and to obtain homogenous antibody preparation. Antibodies recognize different conformations of alpha-synuclein and can be used in a variety of immunochemical approaches, including immunocytochemistry.
Barinova K.V., Melnikova A.K., Schmalhausen E.V., Muronetz V.I. (2018) A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein. Biomeditsinskaya Khimiya, 64(3), 276-282.
Barinova K.V. et al. A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein // Biomeditsinskaya Khimiya. - 2018. - V. 64. -N 3. - P. 276-282.
Barinova K.V. et al., "A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein." Biomeditsinskaya Khimiya 64.3 (2018): 276-282.
Barinova, K. V., Melnikova, A. K., Schmalhausen, E. V., Muronetz, V. I. (2018). A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein. Biomeditsinskaya Khimiya, 64(3), 276-282.
Navarro-Yepes J., Anandhan A., Bradley E., Bohovych I., Yarabe B., de Jong A., Ovaa H., Zhou Y., Khalimonchuk O., Quintanilla-Vega B., Franco R. (2016) Mol. Neurobiol., 53(8), 5229-5251. CrossRef Scholar google search
Xiong Y., Neifert S., Karuppagounder S.S., Stankowski J.N., Lee B.D., Grima J.C., Chen G., Ko H.S., Lee Y., Swing D., Tessarollo L., Dawson T.M., Dawson V.L. (2017) Eneuro, 4(2), ENEURO.0004-17.2017. CrossRef Scholar google search
Beatman E.L., Massey A., Shives K.D., Burrack K.S., Chamanian M., Morrison T.E., Beckham J.D. (2016) J. Virol., 90(6), 2767-2782. CrossRef Scholar google search
Giasson B.I., Jakes R., Goedert M., Duda J.E., Leight S., Trojanowski J.Q., Lee V.M. (2000) J. Neurosci. Res., 59(4), 528-533. CrossRef Scholar google search
