1. Research Centre of Neurology, Moscow, Russia; Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russia 2. Research Centre of Neurology, Moscow, Russia 3. Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russia
Oxidative stress is one of the key factors in brain tissue damage in ischemia, which indicates the appropriateness of using antioxidants under these conditions. One of the promising antioxidants for the therapy of ischemic stroke is the natural dipeptide carnosine. The neuroprotective effect of dietary carnosine administration was investigated in an experimental model of focal cerebral ischemia/reperfusion in Wistar rats. Animals received carnosine with a diet at a daily dose of 150 mg/kg for 7 days before temporary occlusion of the middle cerebral artery (MCA), performed for 60 min. At 24 h after the onset of ischemia the effect of carnosine on the area of the necrotic core was evaluated in animals. In brain tissue of animals the content of malondialdehyde (MDA), protein carbonyls (PC), total antioxidant capacity (TAC), total activity of superoxide dismutase (SOD), glutathione peroxidase (GP), catalase (CAT) and glutathione transferase (GT), content of isoprostanes and cytokines were measured. Carnosine significantly reduced the infarct size. Carnosine also increased TAC and reduced the level of MDA and isoprostanes in brain tissue. Influence of carnosine on other parameters was not detected. Thus carnosine consumed prophylactically with the diet for 7 days before the induction of ischemia by means of MCA occlusion in rats provides the direct neuroprotective effect, retains high antioxidant activity of brain tissue, reduces the level of oxidative damage markers (MDA and isoprostanes) but does not have any effect on the activity of antioxidant enzyme systems and production of cytokines in brain tissue.
Devyatov A.A., Fedorova T.N., Stvolinsky S.L., Ryzhkov I.N., Riger N.A., Tutelyan V.A. (2018) Study of the neuroprotective effects of carnosine in an experimental model of focal cerebral ischemia/reperfusion. Biomeditsinskaya Khimiya, 64(4), 344-348.
Devyatov A.A. et al. Study of the neuroprotective effects of carnosine in an experimental model of focal cerebral ischemia/reperfusion // Biomeditsinskaya Khimiya. - 2018. - V. 64. -N 4. - P. 344-348.
Devyatov A.A. et al., "Study of the neuroprotective effects of carnosine in an experimental model of focal cerebral ischemia/reperfusion." Biomeditsinskaya Khimiya 64.4 (2018): 344-348.
Devyatov, A. A., Fedorova, T. N., Stvolinsky, S. L., Ryzhkov, I. N., Riger, N. A., Tutelyan, V. A. (2018). Study of the neuroprotective effects of carnosine in an experimental model of focal cerebral ischemia/reperfusion. Biomeditsinskaya Khimiya, 64(4), 344-348.
World Health Organization [Internet]. Global Health Estimates 2015: Deaths by Cause, Age, Sex, by Country and by Region, 2000-2015. Geneva, 2016 [cited 2018 Jan 30]. Available from: http://www.who.int/healthinfo/global_burden_disease/estimates/en/index1.html3. Scholar google search
Singhal A.B., Lo E.H., Dalkara T. et al. (2011) in: Acute Ischemic Stroke (González R.G., Hirsch J.A., et al., eds.), Springer-Verlag Berlin Heidelberg, pp. 1-24. Scholar google search
Stvolinskij S.L., Fedorova T.N., Devyatov A.A. i dr. (2017) Zhurnal nevrologii i psikhiatrii im. S.S. Korsakova, 117(12-2), 60-64. CrossRef Scholar google search
Alexandrova M., Bochev P. (2007) in: Oxidative Stress and Neurodegenerative Disorders (Qureshi G, Parvez S., eds.), Elsevier Science, pp. 313-368. CrossRef Scholar google search
Boldyrev A.A., Dobrotvorskaya I.S., Stepanova M.S. et al. (2011) Free Rad. Biol. Med., 51(Suppl), S12. CrossRef Scholar google search
Tripathi T., Pandey R., Raza A. et al. (2010) in: Biomedical Aspects of Histamine (Khardori N., Khan R.A., eds.), Springer, Dordrecht, pp. 421-436. CrossRef Scholar google search
Kalogeris T., Baines C.P., Krenz M. et al. (2016) In: Comprehensive Physiology. Hoboken, NJ, USA: John Wiley & Sons, Inc. pp. 113-170. CrossRef Scholar google search
