Quantitative targeted screening of proteins associated with lung adenocarcinoma cancer by the method of selected reaction monitoring

   
Kopylov A.T.1, Tikhonova O.V.1, Farafonova T.E.1, Novikova S.E.1, Shushkova N.A.1, Shevchenko V.E.2, Liao Pao-Chi3, Archakov A.I.1, Zgoda V.G.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Blokhin Russian Cancer Research Center, Moscow, Russia
3. National Cheng-Kung University, Tainan City, Taiwan
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20186404368      PubMed Id: 30135285
Year: 2018  Volume: 64  Issue: 4  Pages: 368-375
In the present study, we applied selected reaction monitoring (SRM) to a group of proteins that were previously reported to be associated with lung cancer (Novikova S.E. et al. (2017) Biomeditsinskaya khimiya, 63, 181-210. [1]). Measurements were performed on 59 plasma samples. These samples included: 23 samples of plasma of patients diagnosed with lung adenocarcinoma (LAC), 11 samples of plasma of patients diagnosed with squamous cell lung carcinoma (SqCC), 25 samples of donors with no previous history of oncological diseases, and one pooled sample from each of the above group. As a result of the SRM measurements 52 proteins were detected at least in one individual plasma sample. Statistical analysis showed that there were two groups confidently differentiated by the concentration value of 8 proteins wherein 5 proteins displayed increased level (P00738, P26639, P21926, P08603, P51149) in LAC group and 3 proteins (P51884, O15162, Q8N2K0) indicated diminishing the concentration level towards the control level. Data on protein concentrations obtained for LAC and SqCC did not distinguish the samples by statistical clustering analysis. These potential biomarkers can be used for further development of methods for early diagnostics of lung cancer.
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Keywords: lung cancer, lung adenocarcinoma, mass-spectrometry, selected reaction monitoring
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Kopylov, A. T., Tikhonova, O. V., Farafonova, T. E., Novikova, S. E., Shushkova, N. A., Shevchenko, V. E., Liao, Pao-Chi,, Archakov, A. I., Zgoda, V. G. (2018). Quantitative targeted screening of proteins associated with lung adenocarcinoma cancer by the method of selected reaction monitoring. Biomeditsinskaya khimiya, 64(4), 368-375.
This paper is also available as the English translation: 10.1134/S1990750819010062
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