1. Research Institute of Medical Genetics, Tomsk National Research Medical Center, RAS, Tomsk, Russia; Siberian State Medical University, Tomsk, Russia 2. Research Institute of Medical Genetics, Tomsk National Research Medical Center, RAS, Tomsk, Russia 3. Siberian State Medical University, Tomsk, Russia 4. Cardiology Research Institute, Tomsk National Research Medical Center, RAS, Tomsk, Russia
In this study we performed a comparative gene expression analysis of carotid arteries in the area of atherosclerotic plaques and healthy internal mammary arteries of patients with advanced atherosclerosis by using microarray HumanHT-12 BeadChip (“Illumina”). The most down-regulated genes were APOD, FABP4, CIDEC and FOSB, and up-regulated gene was SPP1 (|FC|>64; pFDR<0.05). The majority of differentially expressed genes were down-regulated in advanced atherosclerotic plaques. Unexpectedly, genes involved in immune and inflammatory responses were down-regulated in advanced atherosclerotic plaques to compare with the healthy arteries (arachidonic acid metabolism, cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, Jak-STAT signaling pathway, TNF signaling pathway). “Cellular response to metal ion” (metallothioneins) and “Extracellular matrix organization” were the most significant Gene ontology terms among the down- and up-regulated genes, respectively.
Nazarenko M.S. et al. Comparative analysis of gene expression in vascular cells of patients with advanced atherosclerosis // Biomeditsinskaya Khimiya. - 2018. - V. 64. -N 5. - P. 416-422.
Nazarenko M.S. et al., "Comparative analysis of gene expression in vascular cells of patients with advanced atherosclerosis." Biomeditsinskaya Khimiya 64.5 (2018): 416-422.
Nazarenko, M. S., Markov, A. V., Sleptsov, A. A., Koroleva, I. A., Sharysh, D. V., Zarubin, A. A., Valiahmetov, N. R., Goncharova, I. A., Muslimova, E. F., Kuznecov, M. S., Kozlov, B. N., Afanasiev, S. A., Puzyrev, V. P. (2018). Comparative analysis of gene expression in vascular cells of patients with advanced atherosclerosis. Biomeditsinskaya Khimiya, 64(5), 416-422.
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