Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage

   
Chistyakov D.V.1, Azbukina N.V.1, Goriainov S.V.2, Chistyakov V.V.2, Gancharova O.S.1, Tiulina V.V.1, Baksheeva V.E.1, Iomdina E.N.3, Philippov P.P.1, Sergeeva M.G.1, Senin I.I.1, Zernii E.Yu.4

1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
2. Peoples` Friendship University of Russia, Moscow, Russia
3. Moscow Helmholtz Research Institute of Eye Diseases, Moscow, Russia
4. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20196501033      PubMed Id: 30816095
Year: 2019  Volume: 65  Issue: 1  Pages: 33-40
The ultraviolet (UV) B-induced damage of the eye surface of experimental animals (rabbits) includes loss of corneal epithelium, apoptosis of keratocytes and stromal edema. These changes are accompanied by clinically and histologically manifested corneal inflammation, neutrophil infiltration, and exudation of the anterior chamber of the eye. According to mass spectrometric analysis, UV-induced corneal damage is associated with pronounced changes in the lipid composition of tears, including a decrease in the amount of arachidonic acid and prostaglandin E2 and an increase in the concentrations of prostaglandin D2 and its derivative 15d-PGJ2. In addition, it is accompanied by an alteration in the levels of hydroxyeicosate tetraenic acid derivatives, namely upregulation of 12-HETE and downregulation of 5-HETE. The revealed changes indicate the activation of metabolic pathways involving 5-lipoxygenase, 12-lipoxygenase, cyclooxygenase 1 and 2, and prostaglandin-D-synthase. These findings contribute to understanding mechanisms of UV-induced keratitis and point on feasibility of selective anti-inflammatory therapy for improving corneal regeneration after iatrogenic UV damage.
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Keywords: UV-induced corneal damage, keratocyte apoptosis, keratitis, tear film, eicosanoids, prostaglandin E2, prostaglandin D2, 15d-PGJ2, 5-HETE, 12-HETE
Citation:

Chistyakov, D. V., Azbukina, N. V., Goriainov, S. V., Chistyakov, V. V., Gancharova, O. S., Tiulina, V. V., Baksheeva, V. E., Iomdina, E. N., Philippov, P. P., Sergeeva, M. G., Senin, I. I., Zernii, E. Yu. (2019). Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage. Biomeditsinskaya khimiya, 65(1), 33-40.
This paper is also available as the English translation: 10.1134/S199075081903003X
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