1. Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia 2. Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia; Kemerovo State Medical University, Kemerovo, Russia 3. Siberian State Medical University, Tomsk, Russia 4. Kemerovo Regional Clinical Hospital, Kemerovo, Russia
The purpose of the study was to investigate the features of expression and adiponectin content in the adipocyte culture of subcutaneous, epicardial, and perivascular adipose tissue and the effect of various doses of rosuvastatin on these processes. 29 patients with coronary artery disease were examined. Adipocytes were isolated from the samples of SAT, EAT and PVAT which were taken during coronary artery bypass surgery, followed by cultivation in the presence of rosuvastatin and evaluation of gene expression and adiponectin concentration. Adipocytes SAT, EAT and PVAT differed in the level of adiponectin secretion and expression of its gene. On day 1 of cultivation the expression of the adiponectin gene in the EAT was 2.3 times lower than in the PVAT. On day 2 of cultivation the expression of the adiponectin gene was reduced both in the EAT and the PVAT as compared to the SAT. When rosuvastatin was added at a concentration of 1 mmol/L, adiponectin gene expression in PVAT was higher than when rosuvastatin was added at a concentration of 5 mmol/L, in the adipocyte culture of SAT effect was opposite. Thus, the adipocytes of EZhT and, to a greater extent, PAS, can be a therapeutic target for statins in the case of the pathological activation of adipose tissue.
Dyleva Yu.A., Gruzdeva O.V., Belik E.V., Akbasheva O.E., Uchasova E.G., Borodkina D.A., Sinitsky M.Yu., Sotnikov A.V., Kozyrin K.A., Karetnikova V.N., Barbarash O.L. (2019) Expression of gene and content of adiponectin in fatty tissue in patients with ischemic heart disease. Biomeditsinskaya Khimiya, 65(3), 239-244.
Dyleva Yu.A. et al. Expression of gene and content of adiponectin in fatty tissue in patients with ischemic heart disease // Biomeditsinskaya Khimiya. - 2019. - V. 65. -N 3. - P. 239-244.
Dyleva Yu.A. et al., "Expression of gene and content of adiponectin in fatty tissue in patients with ischemic heart disease." Biomeditsinskaya Khimiya 65.3 (2019): 239-244.
Dyleva, Yu. A., Gruzdeva, O. V., Belik, E. V., Akbasheva, O. E., Uchasova, E. G., Borodkina, D. A., Sinitsky, M. Yu., Sotnikov, A. V., Kozyrin, K. A., Karetnikova, V. N., Barbarash, O. L. (2019). Expression of gene and content of adiponectin in fatty tissue in patients with ischemic heart disease. Biomeditsinskaya Khimiya, 65(3), 239-244.
Ott A.V., Chumakova G.A. (2018) Kompleksnye problemy serdechno-sosudistykh zabolevanij, 7(1), 21-28. Scholar google search
Mahabadi A.A., Berg M.H., Lehmann N., Kalsch H., Bauer M., Kara K., Dragano N., Moebus S., Jöckel K.H., Erbel R., Möhlenkamp S. (2013) J. Am. Coll. Cardiol., 61(13), 1388-1395. CrossRef Scholar google search
Lehr S., Hartwig S., Lamers D., Famulla S., Muller S., Hanisch F.G., Cuvelier C., Ruige J., Eckardt K., Ouwens D.M., Sell H., Eckel J. (2012) Mol. Cell Proteomics, 11(1), M111.010504. CrossRef Scholar google search
Bambacea C., Telescab M., Zoicoa E., Sepe A., Olioso D., Rossi A., Corzato F., Di Francesco V., Mazzucco A., Santini F., Zamboni M. (2011) Cardiovasc. Pathol., 20, E153–E156. Scholar google search
Kadowaki T., Yamauchi T., Kubota N., Hara K., Ueki K., Tobe K. (2006) J. Clin. Invest., 116(7), 1784-1792. CrossRef Scholar google search
Suga H., Matsumoto D., Inoue K., Shigeura T., Eto H., Aoi N., Kato H., Abe H., Yoshimura K. (2008) Plast. Reconstr. Surg., 122(1), 103-114. CrossRef Scholar google search
Wang Z., Schraw T., Kim J., Khan T., Rajala M.W., Follenzi A., Scherer P.E. (2007) Mol. Cell Biol., 27(10), 3716-3731. CrossRef Scholar google search
Tao L., Gao E., Jiao X., Yuan Y., Li S., Christopher T.A., Lopez B.L., Koch W., Chan L., Goldstein B.J., Ma X.L. (2007) Circulation, 115(11), 1408-1416. CrossRef Scholar google search
