The relative differences between post-translational modifications (PTM) of proteins in blood plasma samples of patients with cerebral ischemia (CI) and healthy people were investigated using of the method of label-free comparative proteomic analysis based on the technology of tandem HPLC-MS/MS. For PTM detection we used multiple MS/MS search in the database Mascot for variable PTM and Progenesis LS-MS software. In the CI plasma samples, we observed an increase in the proportion of peptides with such PTM as phosphorylation of serine, threonine, and tyrosine, acetylation of lysine and protein N-term, ubiquitination of lysine and deamidation of glutamine related to clinically significant processes were revealed.
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Keywords: post-translational modifications of proteins, cerebral ischemia, blood plasma proteome, Liquid Chromatography and Mass Spectrometry
Citation:
Kisrieva Y.S., Petushkova N.A., Samenkova N.F., Kuznetsova G.P., Larina O.B., Teryaeva N.B., Usachev D.Yu., Zgoda V.G., Karuzina I.I. (2019) Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method. Biomeditsinskaya Khimiya, 65(3), 251-258.
Kisrieva Y.S. et al. Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method // Biomeditsinskaya Khimiya. - 2019. - V. 65. -N 3. - P. 251-258.
Kisrieva Y.S. et al., "Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method." Biomeditsinskaya Khimiya 65.3 (2019): 251-258.
Kisrieva, Y. S., Petushkova, N. A., Samenkova, N. F., Kuznetsova, G. P., Larina, O. B., Teryaeva, N. B., Usachev, D. Yu., Zgoda, V. G., Karuzina, I. I. (2019). Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method. Biomeditsinskaya Khimiya, 65(3), 251-258.
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