Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method

   
Kisrieva Y.S.1 , Petushkova N.A.1, Samenkova N.F.1, Kuznetsova G.P.1, Larina O.B.1, Teryaeva N.B.2, Usachev D.Yu.2, Zgoda V.G.1, Karuzina I.I.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Burdenko Institute of Neurosurgery, Moscow, Russia
Section: Short Communication
DOI: 10.18097/PBMC20196503251      PubMed Id: 31258150
Year: 2019  Volume: 65  Issue: 3  Pages: 251-258
The relative differences between post-translational modifications (PTM) of proteins in blood plasma samples of patients with cerebral ischemia (CI) and healthy people were investigated using of the method of label-free comparative proteomic analysis based on the technology of tandem HPLC-MS/MS. For PTM detection we used multiple MS/MS search in the database Mascot for variable PTM and Progenesis LS-MS software. In the CI plasma samples, we observed an increase in the proportion of peptides with such PTM as phosphorylation of serine, threonine, and tyrosine, acetylation of lysine and protein N-term, ubiquitination of lysine and deamidation of glutamine related to clinically significant processes were revealed.
Download PDF:
Keywords: post-translational modifications of proteins, cerebral ischemia, blood plasma proteome, Liquid Chromatography and Mass Spectrometry
Citation:

Kisrieva, Y. S., Petushkova, N. A., Samenkova, N. F., Kuznetsova, G. P., Larina, O. B., Teryaeva, N. B., Usachev, D. Yu., Zgoda, V. G., Karuzina, I. I. (2019). Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method. Biomeditsinskaya khimiya, 65(3), 251-258.
References  
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)