1. Institute of Ecology and Genetics of Microorganisms, Ural Branch Russian Academy of Sciences, Perm, Russia; Perm State University, Perm, Russia 2. Institute of Ecology and Genetics of Microorganisms, Ural Branch Russian Academy of Sciences, Perm, Russia 3. Immanuel Kant Baltic Federal University, Kaliningrad, Russia 4. Perm State University, Perm, Russia 5. Institute of Ecology and Genetics of Microorganisms, Ural Branch Russian Academy of Sciences, Perm, Russia; Perm State University, Perm, Russia; Immanuel Kant Baltic Federal University, Kaliningrad, Russia
We studied the effect of the native (non-recombinant) alpha-fetoprotein (AFP) on differentiation, proliferation, and cytokine profile of activated helper T cells 17 (Th17). The object of the study was a culture of isolated by immunomagnetic separation helper T cells (CD4+), induced into the Th17 phenotype by using TCR-activator and proinflammatory cytokines (IL-1β and IL-6). AFP had not significant effect on the frequency of Th17 cells (ROR-γτ+) in the helper T cell culture, and did not affect proliferation of these cells, as measured by Ki-67 expression. Evaluation of the cytokine profile of culture supernatants by using the Luminex xMAP technology, revealed that AFP did not affect the levels of IL-4, IL-5, IL-7, IL-8, IL-10, IL-17, IFN-γ and TNF-α, but at concentrations of 50 IU/ml and 100 IU/ml it increased IL-2 production by activated helper T cells. At the same time, AFP suppressed the synthesis of G-CSF and GM-CSF (10 IU/ml), but stimulated the production of CCL4/MIP-1β (100 IU/ml) and CCL2/MCP-1 chemokines (10 IU/ml and 50 IU/ml).
Zamorina S.A., Timganova V.P., Litvinova L.S., Todosenko N.M., Bochkova M.S., Shardina K.Y., Khramtsov P.V., Rayev M.B., Chereshnev V.A. (2019) The role of alpha-fetoprotein in regulation of the cytokine profile of activated T-helpers and their conversion in Th17 phenotype. Biomeditsinskaya Khimiya, 65(4), 347-355.
Zamorina S.A. et al. The role of alpha-fetoprotein in regulation of the cytokine profile of activated T-helpers and their conversion in Th17 phenotype // Biomeditsinskaya Khimiya. - 2019. - V. 65. -N 4. - P. 347-355.
Zamorina S.A. et al., "The role of alpha-fetoprotein in regulation of the cytokine profile of activated T-helpers and their conversion in Th17 phenotype." Biomeditsinskaya Khimiya 65.4 (2019): 347-355.
Zamorina, S. A., Timganova, V. P., Litvinova, L. S., Todosenko, N. M., Bochkova, M. S., Shardina, K. Y., Khramtsov, P. V., Rayev, M. B., Chereshnev, V. A. (2019). The role of alpha-fetoprotein in regulation of the cytokine profile of activated T-helpers and their conversion in Th17 phenotype. Biomeditsinskaya Khimiya, 65(4), 347-355.
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