Exosomes secretion and autophagy in long-term protection of neurons from excitotoxic damage

Yakovlev A.A.1 , Lyzhin A.A.2, Aleksandrova O.P.2, Khaspekov L.G.2, Gulyaeva N.V.1

1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia; Soloviev Moscow Research and Clinical Center for Neuropsychiatry, Moscow, Russia
2. Brain Research Center at Research Center of Neurology, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20196505361      PubMed Id: 31666406
Year: 2019  Volume: 65  Issue: 5  Pages: 361-365
In the model of induced neuronal resistance to the toxic effect of glutamate (deprivation of trophic factors), exosome secretion is demonstrated. Exosomes are secreted at the development of resistance during deprivation and at the first 24 h after preconditioning, as was shown by dot blot of extracellular fluid using anti-CD63 antibody. The autophagy inhibitor bafilomycin (0.01 μM) significantly reduces the quantity of the secreted exosomes at the stage of autophagy induction and at 24 h after induction. At the same time, inhibition of autophagy during the deprivation of trophic factors prevents the development of resistance, but inhibition of autophagy during the first 24 h after deprivation does not affect the development of resistance. We suggest that the long-term effects of preconditioning may be mediated by exosome secretion.
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Keywords: preconditioning, neurons, deprivation, autophagy, exosomes

Yakovlev, A. A., Lyzhin, A. A., Aleksandrova, O. P., Khaspekov, L. G., Gulyaeva, N. V. (2019). Exosomes secretion and autophagy in long-term protection of neurons from excitotoxic damage. Biomeditsinskaya Khimiya, 65(5), 361-365.
This paper is also available as the English translation: 10.1134/S1990750820010151
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