The effect of a neuroprotective dose of isatin or deprenyl to mice on the profile of brain isatin-binding proteins

Buneeva O.A.1, Kapitsa I.G.2, Ivanova E.A.2, Kopylov A.T.1, Zgoda V.G.1, Medvedev A.E.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Zakusov Institute of Pharmacology, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20196505407      PubMed Id: 31666414
Year: 2019  Volume: 65  Issue: 5  Pages: 407-417
Isatin (indol-2,3-dione), an endogenous biofactor found in the brain, peripheral tissues and biological body fluids of humans and animals, exhibits a wide range of biological and pharmacological activities. They are realized via interaction with numerous isatin-binding proteins. Some of these proteins identified during proteomic profiling of the brain are involved in the development of neurodegenerative pathology. In the context of the neuroprotective effect, the effect of isatin is comparable to the effects of deprenyl (selegiline), a pharmacological agent used for treatment of Parkinson's disease. In this study, we have investigated the effect of a single dose administration of isatin (100 mg/kg) and deprenyl (10 mg/kg) to mice on the profile of the brain isatin-binding proteins. Comparative proteomic analysis of brain isatin-binding proteins of mice treated with isatin or deprenyl resulted in identification of a representative group of proteins (n=200) sensitive to the administration of these substances. The change in the profile of isatin-binding proteins may be obviously attributed to accumulation of isatin and deprenyl in the brain and their interaction with target proteins; this prevents protein binding to the affinity sorbent. Thus identified brain isatin-binding proteins of the control animals obviously represent specific targets that interact directly with isatin (and also with deprenyl) in vivo. Isatin or deprenyl administered to animals interact with these proteins and thus inhibit their binding to the affinity sorbent (immobilized isatin analogue).
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Keywords: isatin, deprenyl, isatin-binding proteins, mouse liver, proteomic profiling
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Buneeva, O. A., Kapitsa, I. G., Ivanova, E. A., Kopylov, A. T., Zgoda, V. G., Medvedev, A. E. (2019). The effect of a neuroprotective dose of isatin or deprenyl to mice on the profile of brain isatin-binding proteins. Biomeditsinskaya Khimiya, 65(5), 407-417.
This paper is also available as the English translation: 10.1134/S1990750820020031
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