Impairment of production and posttranslational changes of placental nuclear and membrane proteins with complicated pregnancy

   
Pogorelova T.N.1 , Gunko V.O.1, Nikashina A.A.1, Mikhelson A.A.1, Alliluev I.A.2, Larichkin A.V.1

1. Scientific-Research Institute of Obstetrics and Pediatrics of Rostov State Medical University, Rostov-on-Don, Russia
2. Scientific-Research Institute of Obstetrics and Pediatrics of Rostov State Medical University, Rostov-on-Don, Russia; Academy of Biology and Biotechnology of the South Federal University, Rostov-on-Don, Russia
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20196506513      PubMed Id: 31876522
Year: 2019  Volume: 65  Issue: 6  Pages: 513-519
The content of nuclear and membrane proteins of the placenta, as well as posttranslational modification of these proteins in physiological pregnancy and placental insufficiency (PI) were studied. Differential centrifugation, electrophoresis in polyacrylamide gel, spectrophotometric methods were used. It was found that with PN there is a decrease in the degree of production of the studied proteins of varying degrees relative to control parameters. For chromatin proteins, a more pronounced decrease in the content of non-histone proteins was found in comparison with histones. Among histone fractions, the maximum decrease was detected in the H2A fraction. The degree of change in the amount of membrane proteins depends on the detergent used. Changes in posttranslational protein modifications disorders are characterized by a decrease in the content of amine and amide (especially difficult to hydrolyze) groups and an increase in carbonyl derivatives of proteins. The revealed changes in the composition and structure of the nuclear and membrane proteins of the placenta, performing numerous regulatory functions, can be triggering links in the chain of molecular damage in the placenta at PI.
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Keywords: nuclear and membrane proteins, placental tissue, placental insufficiency
Citation:

Pogorelova, T. N., Gunko, V. O., Nikashina, A. A., Mikhelson, A. A., Alliluev, I. A., Larichkin, A. V. (2019). Impairment of production and posttranslational changes of placental nuclear and membrane proteins with complicated pregnancy. Biomeditsinskaya khimiya, 65(6), 513-519.
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