1. Novosibirsk State Medical University, Novosibirsk, Russia; Institute of Molecular Biology and Biophysics, Novosibirsk, Russia 2. Novosibirsk State Medical University, Novosibirsk, Russia 3. Institute of Molecular Biology and Biophysics, Novosibirsk, Russia 4. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
In this work, we have compared malignant and non-malignant diseases of the mammary gland using 8 proteins: HRG, MUC1, PAI-1, HSP90αA1, CDH1, ERα, PGR and IL-12. Their concentrations in the supernatants of blood cells and breast biopsies were compared in terms of spontaneous production, induced by a polyclonal activator and after exposure to biopsy samples of the HLDF differentiation factor, as well as the indices of the effect of the polyclonal activator and HLDF on the protein production. In addition, the correlation relationships of the above indicators with the expression of markers of the epithelial-mesenchymal transition: collagen type II (CII), β-1 integrin (CD29) and cadherin-E (CDH1) were studied. The study revealed statistically significant differences in the concentration of HRG in the supernatant of blood cells, IL-12 during spontaneous production by biopsy specimens, PGR production of biopsy specimens induced by the polyclonal activator, CDH1 and IL-12 production biopsy specimens exposed to HLDF. According to the influence index of the polyclonal activator and HLDF, statistically significant differences were found for CDH1production. Comparison of non-specific invasive carcinoma biopsy specimens and non-malignant breast diseases by means of the markers of the epithelial-mesenchymal transition revealed statistically significant differences in CD29 expression and the lack of differences in the expression of CDH1 and CII. This indicates the presence of cell atypia in samples of non-malignant breast diseases; it is confirmed by the recognized correlation between the production of certain proteins and the expression of the epithelial-mesenchymal transition markers.
Keywords: breast diseases, proteins markers, immunohistochemistry
Autenschlyus A.I. et al. Proteins and immunohistochemical markers of breast diseases // Biomeditsinskaya khimiya. - 2020. - V. 66. -N 2. - P. 167-173.
Autenschlyus A.I. et al., "Proteins and immunohistochemical markers of breast diseases." Biomeditsinskaya khimiya 66.2 (2020): 167-173.
Autenschlyus, A. I., Bernado, A. V., Davletova, K. I., Arkhipov, S. A., Zhurakovsky, I. P., Mikhailova, E. S., Proskura, A. V., Bogachuk, A. P., Lipkin, V. M., Lyakhovich, V. V. (2020). Proteins and immunohistochemical markers of breast diseases. Biomeditsinskaya khimiya, 66(2), 167-173.
Torre L.A., Bray F., Siegel R.L., Ferlay J., Lortet-Tieulent J., Jemal A. (2015) CA: A Cancer Journal For Clinicians, 65(2), 87-108. CrossRef Scholar google search
Rolny C., Mazzone M., Tugues S., Laoui D., Johansson I., Coulon C., Squadrito M.L., Segura I., Li X., Knevels E., Costa S., Vinckier S., Dresselaer T., Åkerud P., de Mol M., Salomäki H., Phillipson M., Wyns S., Larsson E., Buysschaert I., Botling J., Himmelreich U., van Ginderachter J.A., de Palma M., Dewerchin M., Claesson-Welsh L., Carmeliet P. (2011) Cancer Cell, 19(1), 31-44. CrossRef Scholar google search