Biomarkers of system inflammation in local and diffuse peritonitis

Mikhalchik E.V.1 , Borodina I.V.1, Vlasova I.V.2, Vakhrusheva T.V.1, Gorbunov N.P.1, Panasenko O.M.1, Titkova S.M.3, Anurov M.V.3, Ivakhov G.B.3, Ermakov I.V.3, Teplyshev A.V.4, Klinov D.V.1

1. Federal Research Clinical Center of Physical Chemical Medicine, FMBA of Russia, Moscow, Russia
2. I.M. Sechenov First Moscow State Medical University (Sechenov University), Institute for Regenerative Medicine, Moscow, Russia
3. Pirogov Russian National Research Medical University, Moscow, Russia
4. Pirogov City Clinical Hospital №1, Moscow, Russia
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20206605411      PubMed Id: 33140736
Year: 2020  Volume: 66  Issue: 5  Pages: 411-418
In cases of any acute surgical abdominal disease the progression of purulent inflammation can lead to local or diffuse peritonitis. The indicators of the degree and specificity of the inflammatory response in blood such as cytokine concentration, neutrophil activity, plasma antioxidant capacity (thiols concentration) could be considered as potential predictors of complications. The luminol-dependent chemiluminescence (CL) response of blood activated by the phorbol ester (PMA), and the concentration of cytokines IL-6, IL-8, IL-10, myeloperoxidase (MPO) and thiols in plasma were measured in patients with uncomplicated condition (group 1, n=8), local peritonitis (group 2, n=9) or diffuse peritonitis (group 3, n=9) at admission to surgery (before surgical operation, b/o), immediately after surgical operation (a/o) and a day after surgery (1 day) as well as in healthy volunteers (norm, n=12). In all time-points the cytokines and MPO concentrations measured by ELISA, in group 3 were higher than in healthy volunteers and in patients in groups 1 and 2. Blood CL demonstrated a more than 5-fold increase above the normal values in all patients, and was also higher in group 2 as compared to group 1 (b/o and a/o). Patients in group 3 had shown both maximum and minimum of CL values, which could be a consequence of neutrophil priming or exhaustion (“immune paralysis”), respectively. The same patients' plasma exhibited low thiol concentration (≤30% vs normal values). In patients with fatal outcomes (group 3, n=2) within a day after surgery, either a decrease of the CL to zero values concurrently with elevated IL-8 and IL-6 concentrations and low thiol levels was observed, or CL exceeded normal values more than 20 times with concurrent complete exhaustion of the plasma thiol pool. No clear dependency between the plasma parameters and neutrophil activity was found. Hence a parameter set for prognosis and/or early diagnosis of infectious complications in acute abdominal pathology should include different biomarkers of the inflammatory response: cytokine profile (IL-6, IL-8, IL-10), MPO and neutrophil activity, antioxidant plasma capacity (e.g., total thiols concentration).
Download PDF:  
Keywords: peritonitis, biomarkers of inflammation, interleukins, myeloperoxidase, chemiluminescence, thiols
Supplementary materials:

Mikhalchik, E. V., Borodina, I. V., Vlasova, I. V., Vakhrusheva, T. V., Gorbunov, N. P., Panasenko, O. M., Titkova, S. M., Anurov, M. V., Ivakhov, G. B., Ermakov, I. V., Teplyshev, A. V., Klinov, D. V. (2020). Biomarkers of system inflammation in local and diffuse peritonitis. Biomeditsinskaya Khimiya, 66(5), 411-418.
This paper is also available as the English translation: 10.1134/S1990750821020074
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)