Comparative study of the human keratinocytes proteome of the HaCaT line: identification of proteins encoded by genes of 18 chromosomes under the influence of detergents

   
Kisrieva Y.S.1 , Samenkova N.F.1, Larina O.B.1, Zgoda V.G.1, Karuzina I.I.1, Rusanov A.L.2, Luzgina N.G.1, Petushkova N.A.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. LLC “Perspective”, Novosibirsk, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20206606469      PubMed Id: 33372905
Year: 2020  Volume: 66  Issue: 6  Pages: 469-476
Using electrospray ionization tandem mass spectrometry, a comparative analysis of the HaCaT keratinocyte proteins encoded by the 18th chromosome was performed before and after exposure to sodium dodecyl sulfate (25 mg/ml) and to Triton X-100 (12.5 mg/ml) in a subtoxic dose for 48 hours. Proteins were identified using the SearchGUI platform (X!Tandem and MS-GF+ search engines). In total, 1284 proteins were found in immortalized human HaCaT keratinocytes and about 75% of them were identified by two or more peptides. Were identified, that 26 proteins were encoded by genes of chromosome 18. Among these proteins, 17 were common for control cells and HaCaT cells treated with SDS. Proteins MARE2 and CTIF were identified only in control keratinocytes. Seven identified proteins encoded by genes of chromosome 18 were found only in detergent-treated keratinocytes: LMAN1, NDUV2, SPB3, VPS4B, KDSR, ROCK1 and RHG28.
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Keywords: human keratinocytes of the HaCaT line, sodium dodecylsulfat, Triton, SearchGUI, X! Tandem, MS-GF+, proteins of the 18th chromosome, HPLC-MS/MS
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Kisrieva, Y. S., Samenkova, N. F., Larina, O. B., Zgoda, V. G., Karuzina, I. I., Rusanov, A. L., Luzgina, N. G., Petushkova, N. A. (2020). Comparative study of the human keratinocytes proteome of the HaCaT line: identification of proteins encoded by genes of 18 chromosomes under the influence of detergents. Biomeditsinskaya khimiya, 66(6), 469-476.
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