The aim of this work was to characterize phenotypically peripheral blood T- and NK lymphocytes expressing an early marker of activation, CD69, and assess the significance of CD69 expression for predicting pregnancy outcome in women with idiopathic reccurent pregnancy loss (IRP) before and after immunocytotherapy (ICT). The study group consisted of 36 patients with IRP who became pregnant after pre-gestational allimmunization, in 30 patients the pregnancy was prolonged to the full term and ended with the birth of a viable baby, in 6 — it was terminated before 12 weeks of gestation. In the control group, 15 fertile women outside pregnancy and 11 women at 12 weeks of physiological pregnancy were examined. Assessment of the CD69 expression in women with prolonged pregnancy revealed the absence of significant differences with the control group in the content and proportion of activated lymphocytes (CD69+). In women with aborted pregnancy after pre-gestational ICT, an increase in the number of almost all analyzed lymphocyte subpopulations responding to the activation stimulus, with a clear tendency to increase the proportion of activated T- but not NK-lymphocytes was found. At 5-6 weeks, the proportion of activated lymphocytes among a subpopulation of cytotoxic T-lymphocytes (CD3+CD8+/CD3+CD8+CD69+) in these women was significantly higher than in women with prolonged pregnancy, which confirms the leading role of effector cytotoxic T-lymphocytes in rejection reactions. Thus, the studies showed the promise of evaluating the expression of the early activation marker CD69 as an additional laboratory criterion for the personable appointment of immunocytotherapy to women with a common reccurent pregnancy loss.
Keywords: reccurent pregnancy loss, immunocytotherapy, expression of CD69, T-lymphocytes, NK-cells
Krechetova L.V. et al. Significance of evaluation of CD69 expression by peripheral blood lymphocytes for predicting pregnancy outcome in women with recurrent pregnancy loss // Biomeditsinskaya khimiya. - 2020. - V. 66. -N 6. - P. 477-484.
Krechetova L.V. et al., "Significance of evaluation of CD69 expression by peripheral blood lymphocytes for predicting pregnancy outcome in women with recurrent pregnancy loss." Biomeditsinskaya khimiya 66.6 (2020): 477-484.
Krechetova, L. V., Vanko, L. V., Vtorushina, V. V., Nikolaeva, M. A., Inviyaeva, E. V., Tetruashvili, N. K. (2020). Significance of evaluation of CD69 expression by peripheral blood lymphocytes for predicting pregnancy outcome in women with recurrent pregnancy loss. Biomeditsinskaya khimiya, 66(6), 477-484.
Miki-Hosokawa T., Hasegawa A., Iwamura C., Shinoda K., Tofukuji S., Watanabe Y., Hosokawa, H., Motohashi S., Hashimoto K., Shirai M., Yamashita M., Nakayama T. (2009) J. Immunol., 183, 8203-8215. CrossRef Scholar google search
Petersen C.C., Nederby L., Roug A.S., Skovbo A., Peterslund N.A., Hokland P., Nielsen B., Hokland M. (2011) Viral Immunol., 24(2),165-169. CrossRef Scholar google search
Martín P., Gómez M., Lamana A., Marín A.M., Cortés J.R., Ramírez-Huesca M., Barreiro O., López-Romero P., Gutiérrez-Vázquez C., de la Fuente H., Cruz-Adalia A., Sánchez-Madrid F. (2010) J. Allergy Clin. Immunol., 126(2), 355-365. CrossRef Scholar google search
Hutnick N.A., Williams N.I., Kraemer W.J., Orsega-Smith E., Dixon R.H., Bleznak A.D., Mastro A.M. (2005) Med. Sci. Sports Exerc., 37(11), 1827-1835. CrossRef Scholar google search
Vega-Ramos J., Alari-Pahissa E., Valle J.D., Carrasco-Marín E., Esplugues E., Borràs M., Martínez A.C., Lauzurica P. (2010) Immunol. Cell Biol., 88(7), 707-715. CrossRef Scholar google search
Labiano S., Meléndez-Rodríguez F., Palazón A., Teijeira Á., Garasa S., Etxeberria I., Aznar M.Á., Sánchez-Paulete A.R., Azpilikueta A., Bolaños E., Molina C., de la Fuente H., Maiso P., Sánchez-Madrid F., de Landázuri M.O., Aragonés J., Melero I. (2017) Oncoimmunology, 6(4), e1283468. CrossRef Scholar google search
Carbone J., Sarmiento E., Gallego A., Lanio N., Navarro J., García S., Fernandez-Cruz E. (2016) J. Reproduct. Immunol., 113, 50-53. CrossRef Scholar google search