The study of tryptophan metabolite concentrations in blood serum and fecal extracts from obese children

   
Shestopalov A.V.1, Shatova O.P.2 , Gaponov A.M.1, Moskaleva N.E.3, Appolonova S.A.3, Tutelyan A.V.4, Makarov V.V.5, Yudin S.M.5, Rumyantsev S.A.6

1. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia; Center for digital and translational Biomedicine “Center for Molecular Health”, Moscow, Russia
2. Pirogov Russian National Research Medical University, Moscow, Russia
3. Sechenov First Moscow State Medical University, Moscow, Russia
4. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, Russia
5. Center for Strategic Planning and Management of Biomedical Health Risks, Moscow, Russia
6. Pirogov Russian National Research Medical University, Moscow, Russia; Center for digital and translational Biomedicine “Center for Molecular Health”, Moscow, Russia
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20206606494      PubMed Id: 33372908
Year: 2020  Volume: 66  Issue: 6  Pages: 494-501
We found that changes in the concentrations of tryptophan metabolites in the blood serum and in the intestinal contents are one of the mechanisms for the formation of metabolic coupling in the system "macroorganism-intestinal microbiota", which undergoes significant changes in the development of obesity. Although blood kynurenine remained basically unchanged in obese children we found an increase in some of its serum metabolites: anthranilic, kynurenic and xanthurenic acids. It is noteworthy that in the analysis of fecal matter in obese children, revealed a 2-fold increase in the level of kynurenine while the concentration of kynurenine pathway metabolites corresponded to the level of the group of healthy children. This may indicate the metabolic activation of the microbiota associated with the intestinal mucosa. This is also supported by the absence of statistically significant differences in the concentration of indole in healthy children and in obese children in fecal analyses, and a significant increase in the concentration of indole-3-lactate and indole-3-acetate in the blood serum of obese children.
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Keywords: microbiota, tryptophan, obesity, kynurenine, indoles, metabolic coupling
Citation:

Shestopalov, A. V., Shatova, O. P., Gaponov, A. M., Moskaleva, N. E., Appolonova, S. A., Tutelyan, A. V., Makarov, V. V., Yudin, S. M., Rumyantsev, S. A. (2020). The study of tryptophan metabolite concentrations in blood serum and fecal extracts from obese children. Biomeditsinskaya khimiya, 66(6), 494-501.
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