Features of the in vitro expression profile of hippocampal neurogenic niche cells during optogenetic stimulation

Khilazheva E.D.1 , Morgun A.V.1, Boytsova E.B.1, Mosiagina A.I.1, Shuvaev A.N.1, Malinovskaya N.A.1, Uspenskaya Yu.A.1, Pozhilenkova E.A.1, Salmina A.B.1

1. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia
Section: Experimental Study
DOI: 10.18097/PBMC20216701034      PubMed Id: 33645520
Year: 2021  Volume: 67  Issue: 1  Pages: 34-41
In the central nervous system of mammals, there are specialized areas in which neurogenesis — neurogenic niches — is observed in the postnatal period. It is believed that astrocytes in the composition of neurogenic niches play a significant role in the regulation of neurogenesis, and therefore they are considered as a promising “target” for the possible control of neurogenesis, including the use of optogenetics. In the framework of this work, we formed an in vitro model of a neurogenic niche, consisting of cerebral endothelial cells, astrocytes and neurospheres. Astrocytes in the neurogenic niche model expressed canalorodopsin ChR2 and underwent photoactivation. The effect of photoactivated astrocytes on the expression profile of neurogenic niche cells was evaluated using immunocytochemical analysis methods. It was found that intact astrocytes in the composition of the neurogenic niche contribute to neuronal differentiation of stem cells, as well as the activation of astroglia expressing photosensitive proteins, changes the expression of molecules characterized by intercellular interactions of pools of resting and proliferating cells in the composition of the neurogenic niche with the participation of NAD+ (Cx43, CD38, CD157), lactate (MCT1). In particular, the registered changes reflect a violation of the paracrine intercellular interactions of two subpopulations of cells, one of which acts as a source of NAD+, and the second as a consumer of NAD+ to ensure the processes of intracellular signal transduction; a change in the mechanisms of lactate transport due to aberrant expression of the lactate transporter MCT1 in cells forming a pool of cells developing along the neuronal path of differentiation. In general, with photostimulation of niche astrocytes, the total proliferative activity increases mainly due to neural progenitor cells, but not neural stem cells. Thus, optogenetic activation of astrocytes can become a promising tool for controlling the activity of neurogenesis processes and the formation of a local proneurogenic microenvironment in an in vitro model of a neurogenic niche.
Download PDF:  
Keywords: neurogenesis, optogenetics, astrocytes, neurogenic niche, neural stem cells

Khilazheva, E. D., Morgun, A. V., Boytsova, E. B., Mosiagina, A. I., Shuvaev, A. N., Malinovskaya, N. A., Uspenskaya, Yu. A., Pozhilenkova, E. A., Salmina, A. B. (2021). Features of the in vitro expression profile of hippocampal neurogenic niche cells during optogenetic stimulation. Biomeditsinskaya Khimiya, 67(1), 34-41.
This paper is also available as the English translation: 10.1134/S1990750821030057
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)