The homodimeric glycoprotein, anti-mullerian hormone (AMH), described over 70 years ago by A. Jost, is the least studied member of the transforming growth factor beta superfamily. Despite the antitumor activity of AMH discovered at the end of the last century, the creation of effective drugs based on AMH is hindered primarily by the lack of information on the mechanism of various AMH forms interaction with a specific type II receptor (MISRII). Previously, we have shown that not only the full-length activated hormone but also its C-terminal fragment (C-rAMH) could bind to MISRII. In this work, using the surface plasmon resonance technique, we compared the interaction of three forms of recombinant AMH (rAMH) with the MISRII analogue — the chimeric protein MISRII-Fc containing AMH type II receptor and a Fc-fragment of the human IgG1 heavy chain. Comparison of the binding of MISRII-Fc, immobilized on a chip with group specificity for human immunoglobulins, to C-rAMH, to intact rAMH (pro-rAMH), and to rAMH containing one uncleaved monomer (hc-rAMH), showed that the KD of the complexes increased: 1.7 nM, 88 nM and 110 nM, respectively. Thus, we have shown that C-terminal fragment of AMH has the maximum affinity for the recombinant MISRII analogue, which indicates the prospects for the development of drugs based on this hormone derivative.
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Keywords: anti-mullerian hormone, AMH type II receptor, protein-protein interaction, surface plasmon resonance, chimeric protein
Citation:
Rak A.Ya., Trofimov A.V., Ischenko A.M., Sokolov A.V. (2021) The study of interaction of different forms of human recombinant anti-mullerian hormone with a chimeric analogue of the AMH type II. Biomeditsinskaya Khimiya, 67(1), 66-73.
Rak A.Ya. et al. The study of interaction of different forms of human recombinant anti-mullerian hormone with a chimeric analogue of the AMH type II // Biomeditsinskaya Khimiya. - 2021. - V. 67. -N 1. - P. 66-73.
Rak A.Ya. et al., "The study of interaction of different forms of human recombinant anti-mullerian hormone with a chimeric analogue of the AMH type II." Biomeditsinskaya Khimiya 67.1 (2021): 66-73.
Rak, A. Ya., Trofimov, A. V., Ischenko, A. M., Sokolov, A. V. (2021). The study of interaction of different forms of human recombinant anti-mullerian hormone with a chimeric analogue of the AMH type II. Biomeditsinskaya Khimiya, 67(1), 66-73.
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