The study of mRNA expression profiles of main cell function regulator genes in unchanged colonic mucosa from healthy donors

   
Zakharenko M.V.1, Bozhenko V.K.1, Kiseleva Ya.Yu.1 , Dzhikiya E.L.1, Stanoevich U.S.2, Kulinich T.M.1, Melnikova N.V.1, Senchukova A.L.1, Urakova A.B.1, Grunin I.B.1, Goncharov S.V.1, Bliznyukov O.P.1, Solodkiy V.A.1

1. Russian Scientific Center of Roentgenoradiology, Moscow, Russia
2. Kursk Regional Clinical Oncology Center, Kislino, Russia
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20216704366      PubMed Id: 34414896
Year: 2021  Volume: 67  Issue: 4  Pages: 366-373
A comparative analysis of molecular genetic phenotypes of mucous membrane cells in five anatomical regions of the colon in a group of healthy donors was conducted by comparing mRNA expression profiles of 62 genes involved in the regulation of vital cellular function. We used 181 biopsy samples of morphologically unchanged colonic mucosa, obtained from the colon (ascending, transverse-colon, descending, sigmoid) and rectum sections during prophylactic colonoscopy of 58 donors with no colon pathology. The mRNA levels for 62 genes involved in the regulation of apoptosis, proliferation, transcription, differentiation, cell-cell adhesion, and immune response were assessed by RT-PCR. Statistically significant differences were found for the molecular phenotypes of five sections of the colon. The results of the study can serve as a basis for creating a reference database (values of expression profiles), developing methods of differential diagnostics and screening of various pathologies of the colon.
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Keywords: gene expression, colon, unchanged colon mucosa, mRNA, RT-PCR
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Zakharenko, M. V., Bozhenko, V. K., Kiseleva, Ya. Yu., Dzhikiya, E. L., Stanoevich, U. S., Kulinich, T. M., Melnikova, N. V., Senchukova, A. L., Urakova, A. B., Grunin, I. B., Goncharov, S. V., Bliznyukov, O. P., Solodkiy, V. A. (2021). The study of mRNA expression profiles of main cell function regulator genes in unchanged colonic mucosa from healthy donors. Biomeditsinskaya khimiya, 67(4), 366-373.
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