PCR analysis of the expression of chromosome 18 genes in human liver tissue: interindividual variability

Timoshenko O.S.1, Khmeleva S.A.1, Poverennaya E.V.1, Kiseleva Y.Y.2, Kurbatov L.K.1, Radko S.P.1 , Buromski I.V.3, Markin S.S.1, Lisitsa A.V.1, Archakov A.I.1, Ponomarenko E.A.1

1. Institute of Biomedical Chemistry, Moscow, Russia
2. Russian Scientific Center of Roentgenoradiology, Moscow, Russia
3. Pirogov Russian National Research Medical University, Moscow, Russia
Section: Clinical and Diagnostic Research
DOI: 10.18097/PBMC20216705418      PubMed Id: 34730555
Year: 2021  Volume: 67  Issue: 5  Pages: 418-426
Using human chromosome 18 (Ch18) genes as an example, a PCR analysis of the interindividual variability of gene expression in liver tissue was performed. Although the quantitative profiles of the Ch18 transcriptome, expressed in the number of cDNA copies per single cell, showed a high degree of correlation between donors (Pearson correlation coefficients ranged from 0.963 to 0.966), the expression of the significant number of genes (from 13% to 19%, depending on the method of experimental data normalization) varied by more than 4-fold when comparing donors pairwise. At the same time, the proportion of differentially expressed genes increased with a decrease in the level of their expression. It is shown that the higher quantitative variability of low-abundance transcripts is mainly not technical, but biological. Bioinformatic analysis of the interindividual variability of the differential expression of chromosome 18 genes in human liver tissue did not reveal any statistically significant groups of genes related to certain biological processes that indicated a rather transient nature of the interindividual variability of their expression, probably reflecting the response of cells of an individual to specific external stimuli.
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Keywords: transcriptome, quantitative PCR, chromosome 18, human liver tissue
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Timoshenko, O. S., Khmeleva, S. A., Poverennaya, E. V., Kiseleva, Y. Y., Kurbatov, L. K., Radko, S. P., Buromski, I. V., Markin, S. S., Lisitsa, A. V., Archakov, A. I., Ponomarenko, E. A. (2021). PCR analysis of the expression of chromosome 18 genes in human liver tissue: interindividual variability. Biomeditsinskaya Khimiya, 67(5), 418-426.
This paper is also available as the English translation: 10.1134/S1990750822010097
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