Influence of N-alkynylaminosteroids on mitochondria function and autophagy in glioma cells

   
Panada J.U.1, Klopava V.A.1, Kulahava T.A.2, Faletrov Y.V.3, Frolova N.S.1, Koran S.V.4, Fomina E.G.4, Shkumatov V.M.3

1. Research Institute for Physical Chemical Problems of the Belarusian State University, Minsk, Belarus
2. Institute for Nuclear Problems of the Belarusian State University, Minsk, Belarus
3. Research Institute for Physical Chemical Problems of the Belarusian State University, Minsk, Belarus; Belarusian State University, Minsk, Belarus
4. Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus
Section: Experimental Study
DOI: 10.18097/PBMC20226802126      PubMed Id: 35485486
Year: 2022  Volume: 68  Issue: 2  Pages: 126-133
In this work we examined the synthesized N-alkynyl-17-aminosteroids and N-alkynyl-20-aminosteroids (based on dehydroepiandrosterone and pregnenolone, respectively) for their effect on C6 rat glioma cell functions. At 10 μM, the compounds had an insignificant effect on C6 glioma mitochondrial membrane potential, but increased cell autophagy by 70-90%, comparable to the known autophagy inducer dexamethasone. Docking simulations predict a potential high-affinity interaction between N-alkynylaminosteroids and Keap1 and the Hedgehog pathway protein, Smoothened, which are involved in autophagy regulation. The possible mechanisms of observed processes are discussed.
Download PDF:
Keywords: glioma, anticancer compounds, alkynylaminosteroids, mitochondria, autophagy
Citation:

Panada, J. U., Klopava, V. A., Kulahava, T. A., Faletrov, Y. V., Frolova, N. S., Koran, S. V., Fomina, E. G., Shkumatov, V. M. (2022). Influence of N-alkynylaminosteroids on mitochondria function and autophagy in glioma cells. Biomeditsinskaya khimiya, 68(2), 126-133.
References  
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)