Although the relationship between the amount of vitamin B₁₂ and the quality of sperm exists, but results are controversial and require several additional research. The objective of our study was to analyse the amount of vitamin B₁₂ in the sperm samples from patients with chronic prostatitis and varicocele with accompanying asthenozoospermia, and to identify the relationship between the amount of vitamin B₁₂ and asthenozoospermia. The research was carried out of men with chronic prostatitis and varicocele with accompanying asthenozoospermia and infertility at the age of 27±2 years. The material of the investigation was spermoplasm. A chemical microscopic examination of the ejaculate was carried out with a sperm analyzer and with the light microscopy. The amount of vitamin B₁₂ in the spermoplasm was determined by the method of competitive ELISA. It was found that the level of vitamin B₁₂ was 3.6 times lower in patients with chronic prostatitis III B and asthenozoospermia then in the control group. Among patients with varicocele of II and III grade and asthenozoospermia, the level of vitamin B₁₂ was 4.4 times lower than in control group. A positive correlation relationship of average strength was revealed (r=0,683; p=0,001). Additionally, it was revealed that among patients with Chronic prostatitis III B and varicocele of II and III grades with accompanying asthenozoospermia, there was the positive correlation relationship of average strength (r=0,690; p=0,001) between the amount of vitamin B₁₂ and sperm mobility. A decrease in vitamin B₁₂ levels may serve as a marker of reproductive dysfunction in men.
Ishtulin A.F., Korotkova N.V., Matveeva I.V., Minaev I.V., Polyakova P.M. (2022) Vitamin B₁₂ is a diagnostic marker of decreased men reproductive function. Biomeditsinskaya Khimiya, 68(3), 228-231.
Ishtulin A.F. et al. Vitamin B₁₂ is a diagnostic marker of decreased men reproductive function // Biomeditsinskaya Khimiya. - 2022. - V. 68. -N 3. - P. 228-231.
Ishtulin A.F. et al., "Vitamin B₁₂ is a diagnostic marker of decreased men reproductive function." Biomeditsinskaya Khimiya 68.3 (2022): 228-231.
Ishtulin, A. F., Korotkova, N. V., Matveeva, I. V., Minaev, I. V., Polyakova, P. M. (2022). Vitamin B₁₂ is a diagnostic marker of decreased men reproductive function. Biomeditsinskaya Khimiya, 68(3), 228-231.
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