The experimental results available in the ProteomeXchange database (accession code PXD016538) (Simats et al. (2020) Molecular & Cellular Proteomics, 19(12), 1921-1936) obtained using a comprehensive multi-omics approach were analyzed in mouse blood to identify potential biomarkers of ischemic stroke. Acetylation, methylation, and ubiquitination were considered as post-translational modifications. The analysis of the significance of changes in the level of protein modification was evaluated for ischemic tissue in comparison with tissue undamaged by stroke and control taken from mice after sham operation. At the level of statistically significant differences according to the Mann-Whitney test (p < 0.05), 2 proteins were found (Q02248 and Q8BL66); for additional 7 proteins, the differences were at the level of a statistical trend (p < 0.1). For 7 of 9 selected proteins there are reports in the literature, for their association with cerebral ischemia.
Skvortsov V.S., Ivanova Y.O., Voronina A.I. (2022) Acetylation, methylation, and ubiquitination of proteins in experimental ischemic stroke in mice: a bioinformatics analysis. Biomeditsinskaya Khimiya, 68(5), 390-397.
Skvortsov V.S. et al. Acetylation, methylation, and ubiquitination of proteins in experimental ischemic stroke in mice: a bioinformatics analysis // Biomeditsinskaya Khimiya. - 2022. - V. 68. -N 5. - P. 390-397.
Skvortsov V.S. et al., "Acetylation, methylation, and ubiquitination of proteins in experimental ischemic stroke in mice: a bioinformatics analysis." Biomeditsinskaya Khimiya 68.5 (2022): 390-397.
Skvortsov, V. S., Ivanova, Y. O., Voronina, A. I. (2022). Acetylation, methylation, and ubiquitination of proteins in experimental ischemic stroke in mice: a bioinformatics analysis. Biomeditsinskaya Khimiya, 68(5), 390-397.
References
Skvortsov V.S., Ivanova Ya.O., Voronina A.I. (2021) The bioinformatic identification of proteins with varying levels of post-translational modifications in experimental ischemic stroke in mice. Biomeditsinskaya khimiya, 67(6), 475-484. CrossRef Scholar google search
Knorre D.G., Kudryashova N.V., Godovikova T.S. (2009) Chemical and Functional Aspects of Posttranslational Modification of Proteins. Acta Naturae, 1(3), 29-51. CrossRef Scholar google search
Kisrieva Y.S., Petushkova N.A., Samenkova N.F., Kuznetsova G.P., Larina O.B., Teryaeva N.B., Usachev D.Yu., Zgoda V.G., Karuzina I.I. (2019) Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method. Biomeditsinskaya khimiya, 65(3), 251-258. CrossRef Scholar google search
Simats A., Ramiro L., García-Berrocoso T., Briansó F., Gonzalo R., Martín L., Sabé A., Gill N., Penalba A., Colomé N., Sánchez A., Canals F., Bustamante A., Rosell A., Montaner J. (2020) A Mouse Brain-based Multi-omics Integrative Approach Reveals Potential Blood Biomarkers for Ischemic Stroke. Molecular and Cellular Proteomics, 19(12), 1921-1936. CrossRef Scholar google search
Ma B., Zhang K., Hendrie C., Liang C., Li M., Doherty-Kirby A., Lajoie G. (2003) PEAKS: powerful software for peptide de novo sequencing by tandem mass spectrometry. Rapid Communications in Mass Spectrometry, 17(20), 2337–2342. CrossRef Scholar google search
The UniProt Consortium (2020) UniProt: the universal protein knowledgebase in 2021. Nucleic Acids Research, 49(D1), D480–D489. CrossRef Scholar google search
Mo Z., Zeng Z., Liu Y., Zeng L., Fang J., Ma Y. (2022) Activation of Wnt/Beta-Catenin Signaling Pathway as a Promising Therapeutic Candidate for Cerebral Ischemia/Reperfusion Injury. Frontiers in Pharmacology, 13, 914537. CrossRef Scholar google search
Song S., Huang H., Guan X., Fiesler V., Bhuiyan M., Liu R., Jalali S., Hasan M.N., Tai A.K., Chattopadhyay A., Chaparala S., Sun M., Stolz D.B., He P., Agalliu D., Sun D., Begum G. (2021) Activation of endothelial Wnt/β-catenin signaling by protective astrocytes repairs BBB damage in ischemic stroke. Progress in neurobiology, 199, 101963. CrossRef Scholar google search
Yuan D., Hu K., Loke C.M., Teramoto H., Liu C., Hu B. (2021) Interruption of endolysosomal trafficking leads to stroke brain injury. Experimental Neurology, 345, 113827. CrossRef Scholar google search
Singh V., Mishra V.N., Chaurasia R.N., Joshi D., Pandey V. (2019) Modes of calcium regulation in ischemic neuron. Indian Journal of Clinical Biochemistry, 34(3), 246-253. CrossRef Scholar google search
Uphaus T., Bittner S., Gröschel S., Steffen F., Muthuraman M., Wasser K., Weber-Krüger M., Zipp F., Wachter R., Gröschel K. (2019) NfL (neurofilament light chain) levels as a predictive marker for long-term outcome after ischemic stroke. Stroke, 50(11), 3077-3084. CrossRef Scholar google search
Kitagawa, K., Matsumoto, M., Sobue, K., Tagaya, M., Okabe, T., Niinobe, M., Ohtsuki T., Handa N., Kimura K., Mikoshiba K., Kamada T. (1992) The synapsin I brain distribution in ischemia. Neuroscience, 46(2), 287-299. CrossRef Scholar google search
Kim J.Y., Kim J.W., Yenari M.A. (2020) Heat shock protein signaling in brain ischemia and injury. Neuroscience letters, 715, 134642. CrossRef Scholar google search
Zhang X., Connelly J., Levitan E.S., Sun D., Wang J.Q. (2021) Calcium/Calmodulin–Dependent Protein Kinase II in Cerebrovascular Diseases. Translational Stroke Research, 12(4), 513-529. CrossRef Scholar google search
