1. Institute of Experimental Medicine, St. Petersburg, Russia; Military Medical Academy of S.M. Kirov, St. Petersburg, Russia 2. Institute of Experimental Medicine, St. Petersburg, Russia; North-Western State Medical University named after I.I. Mechnikov, St. Petersburg, Russia 3. Institute of Experimental Medicine, St. Petersburg, Russia
The microRNA (miR) species analyzed in this study are involved in molecular mechanisms of TLR4 and TLR7 signaling, mediating the development of neuroinflammation and neurodegeneration. We have investigated the expression levels of miR-let7b, miR-96, miR-182, miR-155, and the mRNA content of HMGB1, TLR3, TLR4 in the nucleus accumbens (NAc) of the brain of rats exposed to long-term alcoholization. The long-term alcoholization caused a decrease in miR-let7b, miR-96, miR-182, and TLR7 mRNA levels; this was accompanied by an increase in miR-155, TLR4, and Hmgb1 mRNA levels in the NAc of rat brain. TLR7 is functionally linked to miR-let7b. The data of a simultaneous decrease in miR-let7b and TLR7 mRNA are of interest for further studies; they may indicate on the lack of functionally significant links between Hmgb1 and the miR-let7b-TLR7 system in NAc. The existing evidence of a functional relationship between TLR4 with miR-155 and miR-182 and our observations on their expression changes during chronic alcoholization are very interesting and require further investigation. The suggestion about the development of neuroinflammatory process in NAc under prolonged alcohol exposure are relevant for studying the level of TLR gene expression in NAc, as well as the expression of miR species, which may have a functional relationship with the TLR system.
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Keywords: nucleus accumbens, alcohol, neuroinflammation, TLR, miR
Citation:
Airapetov M.I., Eresko S.O., Shamaeva S.A., Matveev N.M., Bychkov E.R., Lebedev A.A., Shabanov P.D. (2023) Prolonged alcohol consumption influences microRNA expression in the nucleus accumbens of the rat brain. Biomeditsinskaya Khimiya, 69(4), 235-239.
Airapetov M.I. et al. Prolonged alcohol consumption influences microRNA expression in the nucleus accumbens of the rat brain // Biomeditsinskaya Khimiya. - 2023. - V. 69. -N 4. - P. 235-239.
Airapetov M.I. et al., "Prolonged alcohol consumption influences microRNA expression in the nucleus accumbens of the rat brain." Biomeditsinskaya Khimiya 69.4 (2023): 235-239.
Airapetov, M. I., Eresko, S. O., Shamaeva, S. A., Matveev, N. M., Bychkov, E. R., Lebedev, A. A., Shabanov, P. D. (2023). Prolonged alcohol consumption influences microRNA expression in the nucleus accumbens of the rat brain. Biomeditsinskaya Khimiya, 69(4), 235-239.
References
Burov Y.V., Vedernikova N.N. (1985) Neurochemistry and Pharmacology of Alcoholism, Medicine, M. 240 p. Scholar google search
Shabanov P.D., Kalishevich S.Yu. (1998) Biology of Alcoholism, Lan', St. Petersburg, 272 p. Scholar google search
Nestler E.J. (2005) Is there a common molecular pathway for addiction? Nat. Neurosci., 8(11), 1445-1449. CrossRef Scholar google search
Koob G.F. (2014) Neurocircuitry of alcohol addiction: Synthesis from animal models. Handb. Clin. Neurol., 125, 33-54. CrossRef Scholar google search
Edith V.S., Anjali D., Eve R., Margaret J.R., Adolf P. (2005) Striatal and forebrain nuclei volumes: Contribution to motor function and working memory deficits in alcoholism. Biol Psychiatry, 57(7), 768-776. CrossRef Scholar google search
Dahchour A., Ward R.J. (2022) Changes in brain dopamine extracellular concentration after ethanol administration; rat microdialysis studies. Alcohol Alcohol., 57(2), 165-175. CrossRef Scholar google search
Oord E.J.C.G., Xie L.Y., Zhao M., Aberg K.A., Clark S.L. (2023) A single-nucleus transcriptomics study of alcohol use disorder in the nucleus accumbens. Addict. Biol., 28(1), e13250. CrossRef Scholar google search
Sobstyl M., Kupryjaniuk A., Mierzejewski P. (2021) Nucleus accumbens as a stereotactic target for the treatment of addictions in humans: A literature review. Neurol. Neurochir. Pol., 55(5), 440-449. CrossRef Scholar google search
Sheveleva M.V., Lebedev A.A., Roik R.O., Shabanov P.D. (2013) Neurobiological mechanisms of reward and punishment systems in the brain upon activation of the nucleus accumbens. Reviews of Clinical Pharmacology and Drug Therapy, 11(3), 3-19. CrossRef Scholar google search
Lim Y., Beane-Ebel J.E., Tanaka Y., Ning B., Husted C.R., Henderson D.C., Xiang Y., Park I.-H., Farrer L.A., Zhang H. (2021) Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks. Transl. Psychiatry, 11(1), 504. CrossRef Scholar google search
Peregud D.I., Korolkov A.I., Baronets V.Yu., Lobacheva A.S., Arkus M.L., Igumnov S.A., Pirozhkov S.V., Terebilina N.N. (2022) Serum levels of BDNF, miR-30a-5p and miR-122 in the dynamics of alcohol withdrawal syndrome. Biomeditsinskaya Khimiya, 68(3), 218-227. CrossRef Scholar google search
Bayraktar R., Bertilaccio M.T.S., Calin G.A. (2019) The interaction between two worlds: microRNAs and toll-like receptors. Front. Immunol., 10, 1053. CrossRef Scholar google search
Lehmann S.M., Krüger C., Park B., Derkow K., Rosenberger K., Baumgart J., Trimbuch T., Eom G., Hinz M., Kaul D., Habbel P., Kalin R., Franzoni E., Rybak A., Nguyen D., Veh R., Ninnemann O., Peters O., Nitsch R., Heppner F.L., Golenbock D., Schott E., Ploegh H.L., Wulczyn F.G., Lehnardt S. (2012) An unconventional role for miRNA: let-7 activates toll-like receptor 7 and causes neurodegeneration. Nat. Neurosci., 15(6), 827-835. CrossRef Scholar google search
Lippai D., Bala S., Csak T., Kurt-Jones E.A., Szabo G. (2013) Chronic alcohol-induced microRNA-155 contributes to neuroinflammation in a TLR4-dependent manner in mice. PLoS One, 8(8), e70945. CrossRef Scholar google search
Coleman L.G., Zou J., Crews F.T. (2017) Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7. J. Neuroinflammation, 14(1), 22. CrossRef Scholar google search
Ureña-Peralta J.R., Alfonso-Loeches S., Cuesta-Diaz C.M., García-García F., Guerri C. (2018) Deep sequencing and miRNA profiles in alcohol-induced neuroinflammation and the TLR4 response in mice cerebral cortex. Sci. Rep., 8(1), 15913. CrossRef Scholar google search
Ureña-Peralta J.R., Pérez-Moraga R., García-García F., Guerri C. (2020) Lack of TLR4 modifies the miRNAs profile and attenuates inflammatory signaling pathways. PLoS One, 15(8), e0237066. CrossRef Scholar google search
Airapetov M.I., Eresko S.O., Lebedev A.A., Bychkov E.R., Shabanov P.D. (2020) The role of toll-like receptors in the neuroimmunology of alcoholism. Biomeditsinskaya Khimiya, 66(3), 208-215. CrossRef Scholar google search
Shi R., Sun Y.H., Zhang X.H., Chiang V.L. (2012) Poly(T) adaptor RT-PCR. Methods Mol. Biol., 822, 53-66. CrossRef Scholar google search
