In a study on HepG2 cells, the effects of the nitric oxide donor S-nitrosoglutathione on the amount and activity of the polypeptide transporting organic anions, 1B1 (OATP1B1), as well as the expression of the SLCO1B1 gene encoding the transporter protein were studied. In the course of the work, it was found that S-nitrosoglutathione, with a duration of exposure of 3 hours, did not affect the amount and activity of OATP1B1. With an increase in the incubation period to 24 hours, the expression of SLCO1B1 under the action of S-nitrosoglutathione at concentrations of 10, 50 μM by 40 times (p<0.0001), 100 and 500 μM (p=0.0003). The relative amount of OATP1B1 during incubation with Snitrosoglutathione at a concentration of 1 μm did not change, but at concentrations of 10, 50, 100 and 500 μM increased by 136.7% (p=0.0003), 154.1% (p<0.0001), 124.5% (p=0.0006), 131.6% (p=0.0004) accordingly, this was accompanied by an increase in the activity of the transporter protein. The induction of the amount of OATP1B1 was suppressed when a soluble guanylate cyclase inhibitor (rGC) was added to the nutrient medium.Thus, in this study, it was found that S-nitrosoglutathione increases the expression of the SLCO1B1 gene encoding OATP1B1, the relative amount of the transporter protein, and also increases its activity, acting through the NO-rGC-cGMP signaling pathway.
Suchkova O.N., Abalenikhina Yu.V., Shchul'kin A.V., Myl'nikov P.Yu., Gadzhiyeva F.T., Kochanova P.D., Uzbekov M.G., Yakusheva E.N. (2025) Inducing effect of S-nitrosoglutathione on the expression and activity of a polypeptide transporting organic anions 1B1 (OATP1B1) in HepG2 cells. Biomeditsinskaya Khimiya, 70(1), 1-1.
Suchkova O.N. et al. Inducing effect of S-nitrosoglutathione on the expression and activity of a polypeptide transporting organic anions 1B1 (OATP1B1) in HepG2 cells // Biomeditsinskaya Khimiya. - 2025. - V. 70. -N 1. - P. 1-1.
Suchkova O.N. et al., "Inducing effect of S-nitrosoglutathione on the expression and activity of a polypeptide transporting organic anions 1B1 (OATP1B1) in HepG2 cells." Biomeditsinskaya Khimiya 70.1 (2025): 1-1.
Suchkova, O. N., Abalenikhina, Yu. V., Shchul'kin, A. V., Myl'nikov, P. Yu., Gadzhiyeva, F. T., Kochanova, P. D., Uzbekov, M. G., Yakusheva, E. N. (2025). Inducing effect of S-nitrosoglutathione on the expression and activity of a polypeptide transporting organic anions 1B1 (OATP1B1) in HepG2 cells. Biomeditsinskaya Khimiya, 70(1), 1-1.
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