1. Institute of Biomedical Chemistry, Moscow, Russia 2. Institute of Biomedical Chemistry, Moscow, Russia; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia 3. Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia 4. Institute of Biomedical Chemistry, Moscow, Russia; Pirogov Russian National Research Medical University, Moscow, Russia
The interaction of inactivated poliovirus vaccine strains with oriented antibodies immobilized to protein A via Fc fragments has been investigated. Using an SPR biosensor, the kinetic and equilibrium parameters of the interaction of vaccine attenuated polioviruses of the Sabin strains type 1 and type 2, inactivated by various methods were determined. The strongest interaction was observed between polyclonal antibodies to Sabin strain type 2 poliovirus and Sabin strain type 2 poliovirus inactivated with β-propiolactone, KD = 1.04⋅10-11 M, as well as the interaction of monoclonal antibodies to Sabin strain type 1 poliovirus and Sabin strain type 1 poliovirus inactivated with formaldehyde, KD = 1.39⋅10-11 M. The high-affinity interaction of inactivated vaccine polioviruses of the Sabin strains type 1 and type 2 with immobilized antibodies indicates that the D-antigen retained its structure after virus inactivation with β-propiolactone or formaldehyde.
Gnedenko O.V., Ivin Yu.Yu., Piniaeva A.N., Zyrina A.N., Levin I.V., Borisenko N.S., Zhdanov D.D., Ivanov A.S., Lisitsa A.V., Ishmukhametov A.A., Archakov A.I. (2025) The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies. Biomeditsinskaya Khimiya, 71(1), 59-64.
Gnedenko O.V. et al. The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies // Biomeditsinskaya Khimiya. - 2025. - V. 71. -N 1. - P. 59-64.
Gnedenko O.V. et al., "The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies." Biomeditsinskaya Khimiya 71.1 (2025): 59-64.
Gnedenko, O. V., Ivin, Yu. Yu., Piniaeva, A. N., Zyrina, A. N., Levin, I. V., Borisenko, N. S., Zhdanov, D. D., Ivanov, A. S., Lisitsa, A. V., Ishmukhametov, A. A., Archakov, A. I. (2025). The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies. Biomeditsinskaya Khimiya, 71(1), 59-64.
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