Study of the effect of azithromycin on the level of gene expression of the toll-like receptor system in the nucleus accumbens of the rat brain under conditions of ethanol abstinence and search for possible molecular targets by in silico method
1. Institute of Experimental Medicine, St. Petersburg, Russia; Military Medical Academy of S.M. Kirov, St. Petersburg, Russia 2. Institute of Experimental Medicine, St. Petersburg, Russia
Keywords: nucleus accumbens, ethanol, neuroinflammation, toll-like receptor, azithromycin, in silico
Citation:
Airapetov M.I., Eresko S.O., Shchukina A.A., Matveev N.M., Andreev M.A., Bychkov E.R., Lebedev A.A., Shabanov P.D. (2025) Study of the effect of azithromycin on the level of gene expression of the toll-like receptor system in the nucleus accumbens of the rat brain under conditions of ethanol abstinence and search for possible molecular targets by in silico method. Biomeditsinskaya Khimiya, 71(2), 1-1.
Airapetov M.I. et al. Study of the effect of azithromycin on the level of gene expression of the toll-like receptor system in the nucleus accumbens of the rat brain under conditions of ethanol abstinence and search for possible molecular targets by in silico method // Biomeditsinskaya Khimiya. - 2025. - V. 71. -N 2. - P. 1-1.
Airapetov M.I. et al., "Study of the effect of azithromycin on the level of gene expression of the toll-like receptor system in the nucleus accumbens of the rat brain under conditions of ethanol abstinence and search for possible molecular targets by in silico method." Biomeditsinskaya Khimiya 71.2 (2025): 1-1.
Airapetov, M. I., Eresko, S. O., Shchukina, A. A., Matveev, N. M., Andreev, M. A., Bychkov, E. R., Lebedev, A. A., Shabanov, P. D. (2025). Study of the effect of azithromycin on the level of gene expression of the toll-like receptor system in the nucleus accumbens of the rat brain under conditions of ethanol abstinence and search for possible molecular targets by in silico method. Biomeditsinskaya Khimiya, 71(2), 1-1.
References
Shabanov P.D., Lebedev A.A., Meshcherov Sh.K. (2002) Dopamine and Reinforcing Systems of the Brain. Lan', S-Pb, 208 p. Scholar google search
Becker H.C., Mulholland P.J. (2014) Neurochemical mechanisms of alcohol withdrawal. Handb. Clin. Neurol., 125, 133–156. CrossRef Scholar google search
Xu Y., Lin Y., Yu M., Zhou K. (2024) The nucleus accumbens in reward and aversion processing: insights and implications. Front. Behav. Neurosci., 18, 1420028. CrossRef Scholar google search
Yan H., Shlobin N.A., Jung Y., Zhang K.K., Warsi N., Kulkarni A.V., Ibrahim G.M. (2022) Nucleus accumbens: a systematic review of neural circuitry and clinical studies in healthy and pathological states. J. Neurosurg., 138(2), 337–346. CrossRef Scholar google search
López-Gambero A.J., Rodríguez de Fonseca F., Suárez J. (2021) Energy sensors in drug addiction: a potential therapeutic target. Addict. Biol., 26(2), e12936. CrossRef Scholar google search
Dahchour A., Ward R.J. (2022) Changes in brain dopamine extracellular concentration after ethanol administration; rat microdialysis studies. Alcohol Alcohol., 57(2), 165–175. CrossRef Scholar google search
Airapetov M., Eresko S., Lebedev A., Bychkov E., Shabanov P. (2021) The role of Toll-like receptors in neurobiology of alcoholism. Biosci. Trends, 15(2), 74–82. CrossRef Scholar google search
Crews F.T., Coleman L.G. Jr., Macht V.A., Vetreno R.P. (2024) Alcohol, HMGB1, and innate immune signaling in the brain. Alcohol Res., 44(1), 4. CrossRef Scholar google search
Sullivan E.V., Deshmukh A., de Rosa E., Rosenbloom M.J., Pfefferbaum A. (2005) Striatal and forebrain nuclei volumes: contribution to motor function and working memory deficits in alcoholism. Biol. Psychiatry, 57(7), 768–776. CrossRef Scholar google search
Airapetov M., Eresko S., Ignatova P., Lebedev A., Bychkov E., Shabanov P. (2024) Effect of rifampicin on TLR4-signaling pathways in the nucleus accumbens of the rat brain during abstinence of long-term alcohol treatment. Alcohol Alcohol., 59(3), agae016. CrossRef Scholar google search
Kopper T.J., Gensel J.C. (2021) Continued development of azithromycin as a neuroprotective therapeutic for the treatment of spinal cord injury and other neurological conditions. Neural. Regen. Res., 16(3), 508–509. CrossRef Scholar google search
Paxinos G., Watson C. (2017) The Rat Brain in Stereotaxic Coordinates. Academic Press, 160 p. Scholar google search
Way2Drug. Understanding Chemical-Biological Interactions. Predictive services. Retrieved October 14, 2024, from: https://www.way2drug.com/dr/. Scholar google search
Crews F.T., Zou J., Qin L. (2011) Induction of innate immune genes in brain create the neurobiology of addiction. Brain Behav. Immun., 25(1), 4–12. CrossRef Scholar google search
Crews F.T., Vetreno R.P. (2016) Mechanisms of neuroimmune gene induction in alcoholism. Psychopharmacology, 233(9), 1543–1557. CrossRef Scholar google search
Coleman L.G. Jr., Zou J., Qin L., Crews F.T. (2018) HMGB1/IL-1β complexes regulate neuroimmune responses in alcoholism, Brain Behav. Immun., 72, 61–77. CrossRef Scholar google search
Zhang B., Bailey W.M., Kopper T.J., Orr M.B., Feola D.J., Gensel J.C. (2015) Azithromycin drives alternative macrophage activation and improves recovery and tissue sparing in contusion spinal cord injury. J. Neuroinflammation, 12, 218. CrossRef Scholar google search
Gensel J.C., Kopper T.J., Zhang B., Orr M.B., Bailey W.M. (2017) Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment. Sci. Rep., 7, 40144. CrossRef Scholar google search
Amantea D., Certo M., Petrelli F., Bagetta G. (2016) Neuroprotective properties of a macrolide antibiotic in a mouse model of middle cerebral artery occlusion: characterization of the immunomodulatory effects and validation of the efficacy of intravenous administration. Assay Drug Dev. Technol., 14(5), 298–307. CrossRef Scholar google search
Varano G.P., Parisi V., Adornetto A., Cavaliere F., Amantea D., Nucci C., Corasaniti M.T., Morrone L.A., Bagetta G., Russo R. (2017) Post-ischemic treatment with azithromycin protects ganglion cells against retinal ischemia/reperfusion injury in the rat. Molecular Vision, 23, 911–921. Scholar google search
Amantea D., Petrelli F., Greco R., Tassorelli C., Corasaniti M.T., Tonin P., Bagetta G. (2019) Azithromycin affords neuroprotection in rat undergone transient focal cerebral ischemia. Front. Neurosci., 13, 1256. CrossRef Scholar google search
Wu S., Tian X., Mao Q., Peng C. (2023) Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2. Clin. Epigenetics, 15, 12. CrossRef Scholar google search
Tarassishin L., Suh H.-S., Lee S.C. (2011) Interferon regulatory factor 3 plays an anti-inflammatory role in microglia by activating the PI3K/Akt pathway. J. Neuroinflammation, 8, 187. CrossRef Scholar google search
Salvador R., Aridgides D., PereiraPerrin M. (2014) Parasite-derived neurotrophic factor/trans-sialidase of Trypanosoma cruzi links neurotrophic signaling to cardiac innate immune response. Infect. Immun., 82(9), 3687–3696. CrossRef Scholar google search
Thiel F.G., Asgarbeik S., Glaubitz J. (2023) IRAK3-mediated suppression of pro-inflammatory MyD88/IRAK signaling affects disease severity in acute pancreatitis. Sci. Rep., 13, 10833. CrossRef Scholar google search
Kaur N., Ruiz-Velasco A., Raja R., Howell G., Miller J.M., Abouleisa R.R.E., Ou Q., Mace K., Hille S.S., Frey N., Binder P., Smith C.P., Fachim H., Soran H., Swanton E., Mohamed T.M.A., Müller O.J., Wang X., Chernoff J., Cartwright E.J., Liu W. (2022) Paracrine signal emanating from stressed cardiomyocytes aggravates inflammatory microenvironment in diabetic cardiomyopathy. iScience, 25(3), 103973. CrossRef Scholar google search
Xing J., Xu H., Liu C., Wei Z., Wang Z., Zhao L., Ren L. (2019) Melatonin ameliorates endoplasmic reticulum stress in N2a neuroblastoma cell hypoxia-reoxygenation injury by activating the AMPK-Pak2 pathway. Cell Stress Chaperones, 24(3), 621–633. CrossRef Scholar google search
Lebedev K.A., Ponyakina I.D. (2017) Immunology of Image-Recognizing Receptors. Lenand, Moscow, 256 p. Scholar google search
Lin S.J., Kuo M.L., Hsiao H.S., Lee P.T. (2016) Azithromycin modulates immune response of human monocyte-derived dendritic cells and CD4+ T cells. Int. Immunopharmacol., 40, 318–326. CrossRef Scholar google search
Stellari F.F., Sala A., Donofrio G. (2014) Azithromycin inhibits nuclear factor-κB activation during lung inflammation: an in vivo imaging study. Pharmacol. Res. Perspect., 2(5), e00058. CrossRef Scholar google search
Cigana C., Assael B.M., Melotti P. (2007) Azithromycin selectively reduces tumor necrosis factor alpha levels in cystic fibrosis airway epithelial cells. Antimicrob. Agents Chemother., 51(3), 975–981. CrossRef Scholar google search
The Human Protein Atlas. Predictive services. Retrieved October 14, 2024, from: https://www.proteinatlas.org/. Scholar google search