Interaction of antirenalase antibodies with recombinant human renalases 1 and 2 and their C-terminal regions encoded by the alternative exones

  
Fedchenko V.I.1 , Kaloshin A.A.1, Kaloshina S.A.1, Buneeva O.A.1, Kopylov A.T.1, Medvedev A.E.1

1. Institute of Biomedical Chemistry, Moscow, Russia
Section: Experimental Study
DOI: 10.18097/PBMCR1608      PubMed Id: 40904180
Year: 2025  Volume: 71  Issue: 4  Pages: 283-287
The interaction of antirenalase antibodies with full-length recombinant human renalases RNLS1 and RNLS2, as well as fragments of these proteins encoded by alternative exons 9 and 10 and expressed as fusion proteins with dihydrofolate reductase (DHFR) in Escherichia coli cells has been investigated. In this study we used custom made polyclonal antibodies to the full-length recombinant RNLS1 (amino acid residues (aa) 1–342), created at our request, as well as commercially available monoclonal antibodies to the renalase fragment (aa — 18–342), specific for the RNLS1 isoform and its C-terminal sequence encoded by exon 9. According to Western blot analysis, the antibodies interacted not only with recombinant RNLS1 and RNLS2 preparations, but also with fusion proteins containing C-terminal sequences specific for these isoforms (DHFR-RNLS-9ex and DHFR-RNLS-10ex). The results obtained indicate that the studied antibodies, in addition to their direct targets, also “recognized” other protein constructs of RNLS1 and RNLS2, which were absent in the immunogen preparations used for antibody generation.
Download PDF:  
Keywords: polyclonal and monoclonal antirenalase antibodies, human renalase-1 (RNLS1), human renalase-2 (RNLS2), C-terminal regions encoded by alternative exons, fusion proteins DHFR-RNLS-9ex and DHFR-RNLS-10ex
Citation:

Fedchenko, V. I., Kaloshin, A. A., Kaloshina, S. A., Buneeva, O. A., Kopylov, A. T., Medvedev, A. E. (2025). Interaction of antirenalase antibodies with recombinant human renalases 1 and 2 and their C-terminal regions encoded by the alternative exones. Biomeditsinskaya Khimiya, 71(4), 283-287.
References  
 2025 (vol 71)
 2024 (vol 70)
 2023 (vol 69)
 2022 (vol 68)
 2021 (vol 67)
 2020 (vol 66)
 2019 (vol 65)
 2018 (vol 64)
 2017 (vol 63)
 2016 (vol 62)
 2015 (vol 61)
 2014 (vol 60)
 2013 (vol 59)
 2012 (vol 58)
 2011 (vol 57)
 2010 (vol 56)
 2009 (vol 55)
 2008 (vol 54)
 2007 (vol 53)
 2006 (vol 52)
 2005 (vol 51)
 2004 (vol 50)
 2003 (vol 49)