Renalase (RNLS) is a protein that plays an important role in the regulation of blood pressure in humans and animals. Previously, we found higher levels of RNLS mRNA in the hearts of spontaneously hypertensive rats (SHR rats) compared to normotensive controls (WKY — Wistar Kyoto rats) (Fedchenko et al., 2013; Med. Sci. Monit. Basic Res., 19, 267–270). In this study, we assessed the RNLS protein level in the hearts of these animals by means of available poly- and monoclonal antibodies to human and rat RNLS. Western blot analysis revealed an increase in several protein bands within the molecular mass range of RNLS from various sources (35–40 kDa). However, mass spectrometry analysis did not detect the presence of RNLS, and other rat heart proteins were detected in the protein bands of varying intensity: lactate dehydrogenase (LDHB; P42123) and mitochondrial malate dehydrogenase (MDHM; P04636). The interaction of highly purified LDH with anti-RNLS antibodies was confirmed in direct experiments using Western blot analysis. These results indicate that some cardiac proteins can (non-specifically?) interact with anti-RNLS antibodies. This significantly limits their use and requires detailed verification of the obtained results using more specific (alternative) molecular tools.
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Keywords: arterial hypertension, WKY and SHR rats, rat heart, renalase, polyclonal and monoclonal antibodies, Western blotting, mass spectrometry
Citation:
Fedchenko V.I., Buneeva O.A., Kaloshina S.A., Kopylov A.T., Medvedeva M.V., Medvedev A.E. (2026) The use of antibodies to study cardiac renalase levels in the normotensive and hypertensive rats. Biomeditsinskaya Khimiya, 72(2), 112-117.
Fedchenko V.I. et al. The use of antibodies to study cardiac renalase levels in the normotensive and hypertensive rats // Biomeditsinskaya Khimiya. - 2026. - V. 72. -N 2. - P. 112-117.
Fedchenko V.I. et al., "The use of antibodies to study cardiac renalase levels in the normotensive and hypertensive rats." Biomeditsinskaya Khimiya 72.2 (2026): 112-117.
Fedchenko, V. I., Buneeva, O. A., Kaloshina, S. A., Kopylov, A. T., Medvedeva, M. V., Medvedev, A. E. (2026). The use of antibodies to study cardiac renalase levels in the normotensive and hypertensive rats. Biomeditsinskaya Khimiya, 72(2), 112-117.
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