The proteomic composition of a biological sample serves as the most important feature of a biological object, and it allows discriminating normal and pathological conditions. Targeted mass spectrometric analysis, namely, multiple reaction monitoring (MRM) using synthetic isotopically-labeled internal standard (SIS), is the main alternative to the ELISA method for the analysis of diagnostically significant proteins. Based on the MRM results, a prototype test system has been developed; it employs the targeted mass spectrometric method for multiplex, quantitative analysis of FDA-verified proteins in whole blood plasma. Using this approach, it was possible to measure the content of 42 proteins in 31 samples in a concentration range spanning five orders of magnitude. The interindividual variability for 30 of the 42 registered proteins was less than 40%. The largest scatter was observed for haptoglobin (68%), immunoglobulin heavy constant delta IGHD (90%), angiotensin (72%), sex hormone-binding globulin SHBG (100%) and lipoprotein-(a) (136%). The obtained results on the concentration of proteins correlate with published data (Hortin et al., 2008, Clinical Chemistry, 54, 1608) with R2=0.84. The developed prototype test system based on targeted mass spectrometric analysis of proteins can be considered as an alternative to methods using monoclonal antibodies.
Novikova S.E. et al. Mass-spectrometric MRM analysis of FDA-verified proteins in the blood plasma of healthy volunteers // Biomeditsinskaya khimiya. - 2020. - V. 66. -N 4. - P. 294-316.
Novikova S.E. et al., "Mass-spectrometric MRM analysis of FDA-verified proteins in the blood plasma of healthy volunteers." Biomeditsinskaya khimiya 66.4 (2020): 294-316.
Novikova, S. E., Farafonova, T. E., Tikhonova, O. V., Shushkova, N. A., Pyatnitsky, M. A., Zgoda, V. G., Ponomarenko, E. A., Lisitsa, A. V., Grigoryev, A. I., Tutelyan, V. A., Archakov, A. I. (2020). Mass-spectrometric MRM analysis of FDA-verified proteins in the blood plasma of healthy volunteers. Biomeditsinskaya khimiya, 66(4), 294-316.
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