Hexachlorobenzene induction of 2 forms of cytochrome P-450 in the rat liver microsome

Mishin V.M., Gutkina N.I.
PubMed Id: 4002661
Year: 1985  Volume: 31  Issue: 2  Pages: 68-72
Induction of the monooxygenase system of xenobiotic metabolism by means of hexachlorobenzene (HCB) was studied in rat liver microsomes as compared with induction using phenobarbital (PB) and 3-methylcholanthrene (3-MC). HCB was shown to increase the content of cytochrome P-450 as well as aminopyrine- and benzphetamine-N-demethylase activities in liver microsomes. At the same time, HCB increased the benzpyrene hydroxylase activity as it was the case in induction with 3-MC. Electrophoresis in SDS-polyacrylamide gel showed that HCB, as phenobarbital does, induced appearance of the protein with a molecular mass 52,000 (cytochrome P-450) but not of the protein of molecular mass 56,000, which is the main isozyme of cytochrome P-450 in 3-MC microsomes (P-448). Except of cytochrome P-450, cytochrome, immunologically similar to cytochrome-P-448 from 3-MC microsomes, constituted 10% of the total content of cytochrome in HCB microsomes as shown by rocket immunoelectrophoresis using monospecific antibodies towards individual cytochromes P-448 and P-450 (anti-P-448 and anti-P-450). Anti-P-450 and especially anti-P-448 inhibited benzpyrene hydroxylase in HCB microsomes. HCB appears to be an inductor of a 'mixed' type.
Download PDF:  

Mishin, V. M., Gutkina, N. I. (1985). Hexachlorobenzene induction of 2 forms of cytochrome P-450 in the rat liver microsome. Voprosy Meditsinskoi Khimii, 31(2), 68-72.
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)