VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Isolation and purification of L-lysine-alpha-oxidase from Trichoderma sp

   
Khaduev S.Kh., Lukasheva E.V., Smirnova I.P., Berezov T.T.
PubMed Id: 4090359
Year: 1985  Volume: 31  Issue: 5  Pages: 130-134
An improved and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme L-lysyl-alpha-oxidase from Trichoderma sp. The method involves four steps, instead of six steps described previously, with a yield of 22.4%. The purified enzyme preparation was homogeneous as shown by polyacrylamide gel disc electrophoresis and ultracentrifugation. Physico-chemical and antitumor properties of the enzyme are under study.
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Khaduev S.Kh., Lukasheva E.V., Smirnova I.P., Berezov T.T. (1985) Voprosy meditsinskoi khimii, 31(5), 130-134.
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