VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Isolation and purification of lactoperoxidase from cow's milk

   
Denisova I.I., Krasheniuk A.I., Azhitskiĭ G.Yu., Sharaeva T.K., Umovskaia E.A.
PubMed Id: 3953004
Year: 1986  Volume: 32  Issue: 1  Pages: 116-119
A procedure is described for isolation and purification of lactoperoxidase from cow milk. The procedure involved the following steps: isolation of casein from milk, sorption of lactoperoxidase on CM-Sephadex, concentration of the eluate using ultrafiltration, salting out with ammonium sulfate; isoelectric focusing was carried out in the borate-polyol system. Highly purified, active preparation of lactoperoxidase was obtained within a relatively short period by means of the procedure, where the available reagents were used.
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Denisova, I. I., Krasheniuk, A. I., Azhitskiĭ, G. Yu., Sharaeva, T. K., Umovskaia, E. A. (1986). Isolation and purification of lactoperoxidase from cow's milk. Voprosy Meditsinskoi Khimii, 32(1), 116-119.
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