VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Microbiological determination of DNA levels in blood leukocytes

   
Blokh K.O., Paskevich I.F.
PubMed Id: 3082071
Year: 1986  Volume: 32  Issue: 1  Pages: 134-136
A procedure is developed for estimation of DNA microquantities in biological samples, which involved acid hydrolysis of DNA and the following microbiological estimation of its concentration. A sensitivity of the procedure was as little as 0.25 micrograms DNA per ml. At the DNA concentration 0.25-10 micrograms/ml growth of the indicator strain of bacteria exhibited the linear shape. As shown by means of the procedure developed one rat leukocyte contained 6.3 +/- 0.6 X 10(-12) g of DNA.
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Blokh, K. O., Paskevich, I. F. (1986). Microbiological determination of DNA levels in blood leukocytes. Voprosy Meditsinskoi Khimii, 32(1), 134-136.
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